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A total of 62 colon cancer (CC) specimens and paired adjacent normal tissues were collected, and the expression of MYLK\u2010AS1, microRNA (miR)\u2010101\u20105p\/cell division cycle 42 (CDC42) was detected. CC cell lines were transfected with MYLK\u2010AS1, miR\u2010101\u20105p, CDC42\u2010related plasmids, and the biological functions and markers of epithelial\u2010mesenchymal transition (EMT) were analyzed. The binding relationship between MYLK\u2010AS1, miR\u2010101\u20105p, and CDC42 was evaluated. In CC tissues and cell lines, MYLK\u2010AS1 and CDC42 were highly expressed, and miR\u2010101\u20105p was lowly expressed. Inhibition of MYLK\u2010AS1 or upregulation of miR\u2010101\u20105p can inhibit CC cell growth and EMT. miR\u2010101\u20105p inhibited CDC42\/N\u2010wasp axis activation in CC cells by targeting CDC42. Knockdown of CDC42 or upregulation of miR\u2010101\u20105p partially reversed the effects caused by upregulation of MYLK\u2010AS1. MYLK\u2010AS1, which is significantly upregulated in CC, may be a molecular sponge for miR\u2010101\u20105p, and MYLK\u2010AS1 promotes the activation of the CDC42\/N\u2010wasp axis in CC cells by targeting CDC42 through miR\u2010101\u20105p, which in turn promotes tumor development. MYLK\u2010AS1 may be a potential biomarker and target for CC therapy.<\/jats:p>","DOI":"10.1002\/kjm2.12773","type":"journal-article","created":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T11:08:34Z","timestamp":1699700914000},"page":"11-22","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>LncRNA MYLK<\/scp> antisense <scp>RNA<\/scp> 1 activates cell division cycle 42\/Neutal <scp>Wiskott\u2010Aldrich<\/scp> syndrome protein pathway via <scp>microRNA<\/scp>\u2010101\u20105p to accelerate <scp>epithelial\u2010to\u2010mesenchymal transition<\/scp> of colon cancer cells"],"prefix":"10.1002","volume":"40","author":[{"given":"Zhen\u2010Hao","family":"Quan","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Affiliated Hospital of Guangdong Medical University  Zhanjiang Guangdong 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