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Makino. In mice, <jats:italic>G. pentaphyllum<\/jats:italic> extract and gypenoside LXXV have been shown to improve non\u2010alcoholic steatohepatitis (NASH). This study investigated whether gypenoside XIII can regulate lipid accumulation in fatty liver cells or attenuate NASH in mice. We used HepG2 hepatocytes to establish a fatty liver cell model using 0.5\u2009mM oleic acid. Fatty liver cells were treated with different concentrations of gypenoside XIII to evaluate the molecular mechanisms of lipid metabolism. In addition, a methionine\/choline\u2010deficient diet induced NASH in C57BL\/6 mice, which were given 10\u2009mg\/kg gypenoside XIII by intraperitoneal injection. In fatty liver cells, gypenoside XIII effectively suppressed lipid accumulation and lipid peroxidation. Furthermore, gypenoside XIII significantly increased SIRT1 and AMPK phosphorylation to decrease acetyl\u2010CoA carboxylase phosphorylation, reducing fatty acid synthesis activity. Gypenoside XIII also decreased lipogenesis by suppressing sterol regulatory element\u2010binding protein 1c and fatty acid synthase production. Gypenoside XIII also increased lipolysis and fatty acid \u03b2\u2010oxidation by promoting adipose triglyceride lipase and carnitine palmitoyltransferase 1, respectively. In an animal model of NASH, gypenoside XIII effectively decreased the lipid vacuole size and number and reduced liver fibrosis and inflammation. These findings suggest that gypenoside XIII can regulate lipid metabolism in fatty liver cells and improve liver fibrosis in NASH mice. Therefore, gypenoside XIII has potential as a novel agent for the treatment of NASH.<\/jats:p>","DOI":"10.1002\/kjm2.12795","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T14:05:34Z","timestamp":1706709934000},"page":"280-290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Gypenoside <scp>XIII<\/scp> regulates lipid metabolism in <scp>HepG2<\/scp> hepatocytes and ameliorates nonalcoholic steatohepatitis in mice"],"prefix":"10.1002","volume":"40","author":[{"given":"Shu\u2010Chen","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine Chang Gung Memorial Hospital  Taoyuan Taiwan"},{"name":"Graduate Institute of Health Industry Technology, Research Center for Chinese Herbal Medicine Chang Gung University of Science and Technology  Taoyuan City 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