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Human foreskin fibroblasts (HFF\u20101 cells) were stimulated with high glucose (HG). Db\/db mice were utilized to conduct the DFU in vivo model. Cell viability was evaluated using 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\u2010H\u2010tetrazolium bromide assays. Superoxide dismutase activity was determined using detection kits. Reactive oxygen species determination was conducted <jats:italic>via<\/jats:italic> dichlorodihydrofluorescein\u2010diacetate assays. Enzyme\u2010linked immunosorbent assay was used to evaluate 8\u2010oxo\u20107,8\u2010dihydro\u20102\u2032deoxyguanosine levels. Genes and protein expression were analyzed by quantitative real\u2010time polymerase chain reaction, western blotting, or immunohistochemical analyses. Luciferase reporter gene and RNA immunoprecipitation assays determined the interaction with miR\u2010200a\/b\/c\u20103p and GLI family zinc finger protein 2 (GLI2) or ataxia telangiectasia mutated (ATM) kinase. HG repressed cell proliferation and DNA damage repair, promoted miR\u2010200a\/b\/c\u20103p expression, and suppressed ATM and GLI2. MiR\u2010200a\/b\/c\u20103p inhibition ameliorated HG\u2010induced cell proliferation and DNA damage repair repression. MiR\u2010200a\/b\/c\u20103p targeted ATM. Then, the silenced ATM reversed the miR\u2010200a\/b\/c\u20103p inhibition\u2010mediated alleviative effects under HG. Next, GLI2 overexpression alleviated the HG\u2010induced cell proliferation and DNA damage repair inhibition via miR\u2010200a\/b\/c\u20103p. MiR\u2010200a\/b\/c\u20103p inhibition significantly promoted DNA damage repair and wound healing in DFU mice. GLI2 promoted cell proliferation and DNA damage repair by regulating the miR\u2010200\/ATM axis to enhance diabetic wound healing in DFU.<\/jats:p>","DOI":"10.1002\/kjm2.12813","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T15:12:21Z","timestamp":1708614741000},"page":"422-434","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>GLI family zinc finger protein 2<\/scp> promotes skin fibroblast proliferation and <scp>DNA<\/scp> damage repair by targeting the <scp>miR<\/scp>\u2010200\/<scp>ataxia telangiectasia mutated<\/scp> axis in diabetic wound healing"],"prefix":"10.1002","volume":"40","author":[{"given":"Zun\u2010Hong","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Burn &amp; Skin Repair Surgery Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University)  Haikou P.R. 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