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Olfactory mucosa mesenchymal stem cells (OM\u2010MSCs) have promising therapeutic potential in nerve regeneration and functional recovery. This investigation sought to elucidate the functional mechanisms through which exosomes derived from OM\u2010MSCs provide protection against neuroinflammation following SAH. Mouse OM\u2010MSCs and their exosomes were isolated and characterized using various techniques, including transmission electron microscopy, immunofluorescence staining, Western blotting, flow cytometry, and nanoparticle tracking analysis. Hemin\u2010induced HT22 cells were subsequently utilized to assess the impact of OM\u2010MSC\u2010derived exosomes on the inflammatory response, apoptosis, and mitophagy through ELISAs, Western blotting, qPCR, flow cytometry, and immunofluorescence staining. The impacts of exosomes on neuroinflammation and neuronal damage in SAH model mice were assessed using qPCR, ELISAs, Western blotting, immunofluorescence staining, and TUNEL staining. Exosomes derived from OM\u2010MSCs had the capacity to reduce the levels of proinflammatory factors (IL\u20106, IL\u20101<jats:italic>\u03b2<\/jats:italic>, and TNF\u2010<jats:italic>\u03b1<\/jats:italic>) and promote apoptosis in hemin\u2010induced HT22 cells. Exosomes alleviated neuroinflammation and neuronal injury post\u2010SAH, as evidenced by the increase in modified Garcia scores, reduction in the brain water content, decrease in blood\u2013brain barrier permeability, decreases in inflammatory marker levels, and reduction in apoptosis rates. Notably, the protective effects of exosomes derived from OM\u2010MSCs on neuroinflammation and apoptosis, both in vitro and in vivo, were mediated via the activation of mitophagy. These findings provide a fresh perspective for subsequent clinical research in the domain of prevention and treatment strategies.<\/jats:p>","DOI":"10.1002\/kjm2.12951","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T13:04:04Z","timestamp":1739970244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Olfactory mucosa mesenchymal stem cell\u2010derived exosomes protect against neuroinflammation after subarachnoid hemorrhage by activating mitophagy"],"prefix":"10.1002","volume":"41","author":[{"given":"Jian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Haikou Affiliated Hospital of Central South University Xiangya School of Medicine  Haikou Hainan Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of 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