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This study focused on the role of E1A\u2010binding protein p300 (EP300) in the malignant phenotype of HBV\u2010positive HCC cells and its functional mechanism. Increased EP300 expression was detected in HBV\u2010positive tumor tissues and cells compared to their control counterparts. Silencing EP300 reduced tumorigenic activity, proliferation, viability, migration, invasion, and resistance to apoptosis of HBV\u2010positive cells and reduced the concentrations of HBV infection markers HBsAg and HBeAg. These effects were achieved, at least in part, through downregulation of minichromosome maintenance 8 homologous recombination repair factor (MCM8). <jats:italic>MCM8<\/jats:italic> was identified as a target of EP300 and mediated by acetylation modification. MCM8 was upregulated in HBV\u2010positive tumors and HCC cells while decreasing following EP300 silencing in cells. However, the restoration of <jats:italic>MCM8<\/jats:italic> expression in these cells rescued their malignant properties. In summary, this study suggests a role for EP300\u2010mediated <jats:italic>MCM8<\/jats:italic> upregulation in the malignant properties of HBV\u2010positive HCC.<\/jats:p>","DOI":"10.1002\/kjm2.70006","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T16:39:41Z","timestamp":1742229581000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>EP300<\/scp> Modulates <scp>MCM8<\/scp> Transcription and Augments the Malignant Phenotype of Hepatitis B Virus\u2013Positive Hepatocellular Carcinoma Cells"],"prefix":"10.1002","volume":"41","author":[{"given":"Fang","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Liver Disease Infection Suzhou Hospital of Integrated Traditional Chinese and Western Medicine  Suzhou Jiangsu People's Republic of 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