{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T21:29:11Z","timestamp":1788038951286,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study examined YTHDF2's role in modulating IL\u20106R signaling to regulate synovial fibroblast inflammation and bone damage in rheumatoid arthritis (RA). Synovial tissues of RA patients were collected. Human fibroblast\u2010like synoviocyte (FLS), MH7A cell line, was induced with TNF\u2010\u03b1 and transfected. Cell proliferation was assessed using MTT and EdU assays; apoptosis was measured with flow cytometry, and migration and invasion were evaluated through scratch and Transwell assays. Lentiviral vectors designed to overexpress YTHDF2 or IL\u20106R were created to study their effects in mice with collagen\u2010induced arthritis (CIA). Pathological changes of ankle joints in mice were observed, and TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106 contents were determined. MMP3 and MMP9 levels were detected by Western blot, while YTHDF2 and IL\u20106R were detected by RT\u2010qPCR and Western blot. The binding relationship between YTHDF2 and IL\u20106R was studied. YTHDF2 in synovial tissues of RA patients was down\u2010regulated. Elevating YTHDF2 inhibited TNF\u2010\u03b1\u2010induced MH7A cell proliferation, migration, invasion, and pro\u2010inflammatory factors; Knocking down YTHDF2 showed the opposite effect. Upregulating YTHDF2 improved synovial inflammation and bone damage in CIA mice. IL\u20106R in synovial tissues of patients was significantly up\u2010regulated and negatively correlated with YTHDF2 expression. YTHDF2 reduced IL\u20106R mRNA stability in a m6A\u2010dependent manner. Overexpressing IL\u20106R impaired the anti\u2010proliferating and anti\u2010inflammatory effect of YTHDF2 on TNF\u2010\u03b1\u2010induced MH7A cells. In CIA mice, overexpression of IL\u20106R reversed the benefits on synovial inflammation and bone injury mediated by up\u2010regulating YTHDF2. YTHDF2 inhibits inflammation and bone damage in RA synovial fibroblasts by reducing the mRNA stability of IL\u20106R.<\/jats:p>","DOI":"10.1002\/kjm2.70010","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T07:39:39Z","timestamp":1750664379000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>RNA<\/scp>\n                    Binding Protein\n                    <scp>YTHDF2<\/scp>\n                    Inhibits Synovial Fibroblast Inflammation and Bone Injury in Rheumatoid Arthritis by Reducing the\n                    <scp>mRNA<\/scp>\n                    Stability of\n                    <scp>IL<\/scp>\n                    \u2010\n                    <scp>6R<\/scp>"],"prefix":"10.1002","volume":"41","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology People's Hospital of Ningxia Hui Autonomous Region (The Third Affiliated Hospital of Ningxia Medical University)  Yinchuan City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu\u2010Qing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Neurology People's Hospital of Ningxia Hui Autonomous Region (The Third Affiliated Hospital of Ningxia Medical University)  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Li","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology People's Hospital of Ningxia Hui Autonomous Region (The Third Affiliated Hospital of Ningxia Medical University)  Yinchuan City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin\u2010Yan","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology People's Hospital of Ningxia Hui Autonomous Region (The Third Affiliated Hospital of Ningxia Medical University)  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