{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:58:27Z","timestamp":1753869507910,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901510"],"award-info":[{"award-number":["81901510"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015401","name":"Key Research and Development Projects of Shaanxi Province","doi-asserted-by":"publisher","award":["2023\u2010YBSF\u2010496"],"award-info":[{"award-number":["2023\u2010YBSF\u2010496"]}],"id":[{"id":"10.13039\/501100015401","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Trophoblast cells are specialized placental epithelial cells essential for pregnancy maintenance. miR\u2010152 is implicated in trophoblast cell regulation and pregnancy failure. This study explores the role of miR\u2010152 in decidual natural killer (dNK) cell viability and trophoblast cell invasion. HTR\u20108\/SVneo cells were transfected with miR\u2010152\u2010mimics\/inhibitor or their respective controls, followed by co\u2010culture with dNK cells. RT\u2010qPCR assessed transfection efficiency, while cytokine secretion (IL\u20108, IP\u201010, VEGF), cell viability, apoptosis, and invasion were evaluated via ELISA, CCK\u20108, flow cytometry, Western blot, and Transwell assays. The interaction between miR\u2010152 and HLA\u2010G was examined via dual\u2010luciferase reporter assay, and HLA\u2010G\/sHLA\u2010G levels were measured. Co\u2010cultures of dNK cells and miR\u2010152\/HLA\u2010G\u2010overexpressing HTR\u20108\/SVneo cells were established, and anti\u2010KIR2DL4\/IgG1 was used to block HLA\u2010G\/KIR2DL4 binding. Co\u2010immunoprecipitation confirmed protein interactions. miR\u2010152 overexpression suppressed dNK cell cytokine secretion, reduced HTR\u20108\/SVneo cell viability and invasion, and promoted apoptosis. miR\u2010152 inhibition had the opposite effect. miR\u2010152 directly targeted HLA\u2010G, and HLA\u2010G overexpression rescued dNK function and trophoblast invasion. Blocking the HLA\u2010G\/KIR2DL4 binding counteracted the effects of miR\u2010152. miR\u2010152 inhibits dNK cell function and trophoblast invasion by targeting HLA\u2010G, reducing HLA\u2010G\/KIR2DL4 interaction. These findings highlight a potential regulatory mechanism in pregnancy maintenance.<\/jats:p>","DOI":"10.1002\/kjm2.70019","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T11:34:59Z","timestamp":1746099299000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of <scp>microRNA<\/scp>\u2010152 Regulating Decidual Natural Killer Cell Viability and Affecting Trophoblast Cell Invasiveness via the <scp>HLA<\/scp>\u2010G\/<scp>KIR2DL4<\/scp> Axis"],"prefix":"10.1002","volume":"41","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center Xi'an People's Hospital (Xi'an Fourth Hospital)  Xi'an P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sai","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Xi'an People's Hospital (Xi'an Fourth Hospital)  Xi'an P. R. 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