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Immunohistochemical staining and quantitative reverse transcription PCR (qRT\u2010PCR) validated these findings in clinical specimens and cell lines. Functional characterization of RASD2 was performed through loss\u2010of\u2010function studies, examining cellular proliferation, invasion, and glycolytic parameters. The prognostic significance of RASD2 was evaluated through Kaplan\u2013Meier analysis. Using integrated bioinformatic approaches, we identified miRNA\u2010485\u20105p as a potential RASD2 regulator and confirmed this interaction through molecular studies. The therapeutic potential of targeting RASD2 was assessed using xenograft and pulmonary metastasis models. RASD2 showed significant upregulation in thyroid cancer tissues, with elevated expression correlating with adverse clinicopathological parameters including lymphatic metastasis, extrathyroidal invasion, and advanced TNM stage. Genetic silencing of RASD2 in IHH4 and TPC\u20101 cells substantially impaired their malignant phenotypes, manifesting as decreased proliferation, invasion, and glycolytic activity. Mechanistically, we identified miRNA\u2010485\u20105p as a crucial negative regulator of RASD2, whose overexpression recapitulated the tumor\u2010suppressive effects of RASD2 knockdown. In\u00a0vivo studies further validated the therapeutic potential of RASD2 inhibition, demonstrating reduced tumor growth and metastatic burden. Our findings establish the miRNA\u2010485\u20105p\/RASD2 axis as a critical regulatory pathway in thyroid cancer progression, offering new insights into disease pathogenesis and potential therapeutic interventions.<\/jats:p>","DOI":"10.1002\/kjm2.70028","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T08:32:13Z","timestamp":1745829133000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Modulation of <scp>RASD2<\/scp> by <scp>miRNA<\/scp>\u2010485\u20105p Drives Thyroid Cancer Progression and Metastasis"],"prefix":"10.1002","volume":"41","author":[{"given":"Xiao\u2010Yu","family":"Li","sequence":"first","affiliation":[{"name":"Department of Thyroid and Breast Surgery The Second Hospital of Hebei Medical University  Shijiazhuang 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