{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:52Z","timestamp":1786978132985,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274538"],"award-info":[{"award-number":["82274538"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MH357"],"award-info":[{"award-number":["ZR2020MH357"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study was aimed to elucidate the cytotoxic effects of \u03b3\u03b4T cells on triple\u2010negative breast cancer (TNBC) cells and assess their antitumor efficacy in a mouse xenograft model. Furthermore, the underlying mechanisms of \u03b3\u03b4T cell action on TNBC were explored. The study utilized three TNBC cell lines (MDA\u2010MB\u2010231, MDA\u2010MB\u2010468, and BT\u2010549) as target cells, with \u03b3\u03b4T cells serving as effector cells. Cytotoxicity was assessed in different effector\u2010to\u2010target ratios (E:T) at 5:1, 10:1, and 20:1 subsequent to coculture. To evaluate the antitumor effects of \u03b3\u03b4T cells in\u00a0vivo, a xenograft mice model was established by inoculating MDA\u2010MB\u2010231 cells into the mammary fat pad of B\u2010NDG mice. The mice received tail vein injections of \u03b3\u03b4T cells at different doses. The effects on tumor growth, mouse body weight, and \u03b3\u03b4T cell accumulation in the spleen were then determined. \u03b3\u03b4\u03a4 cells at E:T of 10:1 exhibited significant cytotoxicity against all three TNBC cell lines, indicating a statistically significant difference compared to the control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). The cytotoxic effect at this ratio was superior to that at 20:1 and 5:1 effector\u2010to\u2010target ratios, as evidenced by statistical significance (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Following 21\u2009days of adoptive transfer via tail vein injection, \u03b3\u03b4\u03a4 cells at both low and high doses significantly reduced tumor volume and mass compared to the PBS control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). This reduction was accompanied by an increased accumulation of \u03b3\u03b4\u03a4 cells in the spleen. In conclusion, \u03b3\u03b4\u03a4 cells exert significant cytotoxic effects on TNBC cells and effectively inhibit the growth of breast cancer xenografts in mice while also promoting the accumulation of \u03b3\u03b4\u03a4 cells in the mouse spleen.\n                  <\/jats:p>","DOI":"10.1002\/kjm2.70029","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T04:25:57Z","timestamp":1745814357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Preliminary Study on the Therapeutic Role of\n                    <scp>\u03b3\u03b4T<\/scp>\n                    Cells in Triple\u2010Negative Breast Cancer"],"prefix":"10.1002","volume":"41","author":[{"given":"Shi","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Oncology Affiliated Hospital of Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin\u2010Yu","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Breast Center The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Breast Center The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Breast Center The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7106-9473","authenticated-orcid":false,"given":"Xiang\u2010Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Breast Center The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14051253"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2021.09.019"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.06.005"},{"issue":"1","key":"e_1_2_7_5_1","first-page":"37","article-title":"Treatment Outcomes and Clinicopathologic Characteristics of Triple\u2010Negative Breast Cancer: A Report From Cancer Institute of Iran","volume":"11","author":"Mirzania M.","year":"2017","journal-title":"International Journal of Hematology\u2010Oncology and Stem Cell Research"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djz208"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.5046"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109444"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2022.07.024"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1809615"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1910549"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31962-0"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2019.11.007"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01341-0"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2021.03.011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.31272"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.600573"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.844142"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17414-y"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.1095839"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1302587"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.610303"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.72534"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.03.027"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.894315"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30109"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01305"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1420107"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.07.046"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2020.12188"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.breast.2017.12.017"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28217"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0515-7"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-3037"}],"container-title":["The Kaohsiung Journal of Medical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/kjm2.70029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T04:27:25Z","timestamp":1755750445000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/kjm2.70029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,28]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/kjm2.70029"],"URL":"https:\/\/doi.org\/10.1002\/kjm2.70029","archive":["Portico"],"relation":{},"ISSN":["1607-551X","2410-8650"],"issn-type":[{"value":"1607-551X","type":"print"},{"value":"2410-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,28]]},"assertion":[{"value":"2024-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70029"}}