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Furthermore, continuous subcutaneous infusion of Serelaxin (0.5\u2009mg\/kg\/day; human recombinant relaxin\u20102) improved neurological recovery, as evidenced by higher Basso\u2010Beattie\u2010Bresnahan (BBB) scores and reduced foot\u2010stepping angles compared to the SCI group. Additionally, RLN\u20102 effectively reduced edema in the injured spinal cord, as shown by decreased water content and downregulated AQP4 expression at mRNA and protein levels. RLN\u20102 reduced oxidative stress markers such as malondialdehyde (MDA) and reactive oxygen species (ROS) and increased the activity of catalase (CAT). Further, RLN\u20102 mitigated neuroinflammation by reducing the levels of pro\u2010inflammatory cytokines (TNF\u2010\u03b1 and IL\u20106) and by inhibiting the activation of M1 microglia while promoting the polarization of M2 microglia. It also inhibited the activation of the NF\u2010\u03baB signaling and strengthened the activation of the STAT6 signaling in the spinal cord of SCI mice. These findings suggest that RLN\u20102 may be a promising therapeutic agent for the treatment of spinal cord injury.<\/jats:p>","DOI":"10.1002\/kjm2.70041","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T09:15:18Z","timestamp":1747732518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Relaxin\u20102 Ameliorates Spinal Cord Injury by Inhibiting Microglia Activation"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3995-6395","authenticated-orcid":false,"given":"Ji\u2010Huan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Fengcheng Hospital of Fengxian District  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Biao","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Fengcheng Hospital of Fengxian 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