{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:39:03Z","timestamp":1779237543416,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ulcerative colitis (UC) has become a prevalent global health concern. This study scrutinized the influence of Astragaloside IV (ASI) on DSS\u2010induced UC, with particular emphasis on the role of WDR5 in mediating ENO1 expression. The therapeutic efficacy of ASI was assessed in a mouse model of UC by evaluating disease activity index, pathology, colon length, and inflammatory factor contents. Through bioinformatics analysis, the UC\u2010related differentially expressed genes were predicted using the GSE38713 database and intersected with the lists of ASI targets and transcription factors, and the protein\u2013protein network was constructed to screen the key target transcription factors. ASI inhibited the shortening of colon length, reduced histological damage scores, ameliorated pathology, and the overproduction of pro\u2010inflammatory cytokines. After ASI treatment, DSS\u2010stimulated human NCM460 cells showed increased cell viability, decreased levels of pro\u2010inflammatory cytokines and cleaved\u2010Caspase\u20103, and enhanced ZO\u20101 and claudin\u20103 expression. WDR5 was a target of ASI in UC, and overexpression of WDR5 compromised the effects of ASI. WDR5 promoted the H3K4me3 modification of the ENO1 promoter and thereby regulated ENO1 transcriptional activation. Silencing of ENO1, again, repressed NCM460 cell apoptosis and alleviated UC\u2010like symptoms in mice. In conclusion, ASI mitigated UC by inhibiting WDR5 and reducing H3K4me3\u2010mediated ENO1 activation.<\/jats:p>","DOI":"10.1002\/kjm2.70064","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T09:10:41Z","timestamp":1751274641000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Astragaloside\n                    <scp>IV<\/scp>\n                    Alleviates Ulcerative Colitis Progression by Inhibiting\n                    <scp>WDR5<\/scp>\n                    \u2010Mediated\n                    <scp>ENO1\u00a0H3K4me3<\/scp>\n                    Modification"],"prefix":"10.1002","volume":"41","author":[{"given":"Su\u2010Xiao","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Suzhou Hospital of Integrated Traditional Chinese and Western Medicine  Suzhou Jiangsu People's Republic of 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