{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:15:06Z","timestamp":1790792106480,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Periodontal ligament stem cells (PDLSCs) are derived from periodontal tissue and can differentiate into osteoblasts, which are ideal materials for alveolar bone repair and periodontal tissue regeneration. In this study, we aimed to explore the effects of Y\u2010box binding protein 1 (YB\u20101) on the osteogenic differentiation and proliferation of PDLSCs and its underlying mechanism. hPDLSC proliferation was detected by CCK\u20108 and EdU assays. The osteogenic differentiation of hPDLSCs was clarified using alkaline phosphatase (ALP) activity detection and Alizarin red S (ARS) staining. The expression levels of osteogenic differentiation\u2010related factors, Akt\/GSK3\u03b2\/\u03b2\u2010catenin pathway\u2010related factors, YB\u20101, and fibroblast growth factor 2 (FGF2) were evaluated using qPCR and Western blotting. The interplay between YB\u20101 and FGF2 was clarified using ChIP and dual\u2010luciferase reporter gene assays. YB\u20101 expression was markedly decreased in periodontitis clinical tissues but increased in hPDLSCs during osteogenic differentiation. Moreover, silencing YB\u20101 suppressed the osteogenic differentiation and proliferation of hPDLSCs. In addition, YB\u20101 promotes hPDLSC proliferation and osteogenic differentiation in a manner dependent on the activation of the Akt\/GSK3\u03b2\/\u03b2\u2010catenin signaling pathway, which is mediated by FGF2 transcriptional activation. Furthermore, the inhibitory effects of YB\u20101 knockdown on the osteogenic differentiation and proliferation of hPDLSCs were antagonized by human recombinant FGF2. Taken together, our findings revealed that YB\u20101 facilitates hPDLSC proliferation and osteogenic differentiation through increasing the level of FGF2 via transcriptional regulation, thereby activating the Akt\/GSK3\u03b2\/\u03b2\u2010catenin pathway.<\/jats:p>","DOI":"10.1002\/kjm2.70079","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:24:33Z","timestamp":1753881873000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Y\u2010Box\u2010Binding Protein 1 Facilitates the Proliferation and Osteogenic Differentiation of Periodontal Ligament Stem Cells Through the Transcriptional Activation of\n                    <scp>FGF2<\/scp>\n                    \u2010Mediated Akt\/\n                    <scp>GSK3\u03b2<\/scp>\n                    \/\u03b2\u2010Catenin Signaling"],"prefix":"10.1002","volume":"41","author":[{"given":"Yun\u2010Hao","family":"Xi","sequence":"first","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Department of Stomatology Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Shun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Stomatology The Affiliated Stomatological Hospital of Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin\u2010Lin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Department of Stomatology Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Stomatology Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing China"},{"name":"Department of Orthopedics and Traumatology Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian\u2010Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Department of Stomatology Jiangsu Province Academy of Traditional Chinese Medicine  Nanjing 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