{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:42:02Z","timestamp":1790109722405,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004694","name":"Kaohsiung Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004694","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011645","name":"Kaohsiung Medical University Chung-Ho Memorial Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011645","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Metabolic dysfunction\u2010associated steatotic liver disease (MASLD) is an increasingly prevalent chronic liver condition that can progress to severe complications such as metabolic dysfunction\u2010associated steatohepatitis (MASH). Despite its growing burden, there are no reliable non\u2010invasive biomarkers for tracking disease progression. In this study, we established a murine MASLD\/MASH model using a high\u2010fat diet and chemical (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) induction. We analyzed serum\u2010derived extracellular vesicles (EVs) at 14 and 28\u2009weeks to identify stage\u2010specific proteomic signatures. Proteomic profiling of circulating EVs revealed key proteins associated with disease progression, including cathepsin B (Ctsb) and prosaposin (Psap) in early MASLD, and coagulation factor XIII A chain (F13a1) and polymeric immunoglobulin receptor (Pigr) in early MASH. The significant and severe MASH stages notably enriched Psma2, Psmb3, and Psmb5. These findings suggest EV\u2010associated proteins may be promising non\u2010invasive biomarkers for differentiating MASLD\/MASH stages and guiding clinical monitoring.\n                  <\/jats:p>","DOI":"10.1002\/kjm2.70107","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T12:27:41Z","timestamp":1757420861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Proteomic Profiling of Circulating Extracellular Vesicles of Western Diet and Chemical\u2010Induced Murine\n                    <scp>MASH<\/scp>\n                    Model"],"prefix":"10.1002","volume":"42","author":[{"given":"Szu\u2010Jen","family":"Wang","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine Kaohsiung Medical University  Kaohsiung Taiwan"},{"name":"Division of Hepatogastroenterology, Department of Internal Medicine Shin Huey Shin Hospital  Kaohsiung 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College of Medicine and Center of Excellence for Metabolic Associated Fatty Liver Disease National Sun Yat\u2010sen University  Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"En\u2010Sheng","family":"Lin","sequence":"additional","affiliation":[{"name":"Hepatitis Research Center, College of Medicine; Center for Metabolic Disorders and Obesity; Center for Liquid Biopsy and Cohort Research Kaohsiung Medical University  Kaohsiung Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology Kaohsiung Medical University  Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Shiun","family":"Chen","sequence":"additional","affiliation":[{"name":"Hepatitis Research Center, College of Medicine; Center for Metabolic Disorders and Obesity; Center for Liquid Biopsy and Cohort Research Kaohsiung Medical University  Kaohsiung Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology Kaohsiung 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