{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T03:38:26Z","timestamp":1775187506816,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study explores the regulatory mechanism of the miR\u2010128\u20103p in diabetic retinopathy (DR)\u2010associated inflammatory injury. A cellular model of DR was established by inducing immortalized human retinal endothelial cells (IM\u2010HRECs) with high\u2010glucose (HG). Cell viability was evaluated by CCK\u20108 assay, and the levels of TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u201010 were measured by ELISA. RT\u2010qPCR was performed to determine miR\u2010128\u20103p expression, and miR\u2010128\u20103p mimics were transfected into cells to verify its regulatory role in DR\u2010associated inflammatory injury. miR\u2010128\u20103p was predicted by Starbase to bind to the 3\u2032 UTR of KDM3A, which was verified by dual\u2010luciferase assay. The expressions of KDM3A and NLRP3 in cells were examined by Western blotting, and the enrichment of KDM3A and H3K9me2 on the NLRP3 promoter was measured by Ch\u2010IP assay. The results revealed that HG treatment significantly reduced both IM\u2010HREC viability and IL\u201010 levels, increased the levels of TNF\u2010\u03b1 and IL\u20101\u03b2, and downregulated the expression of miR\u2010128\u20103p. Overexpression of miR\u2010128\u20103p reduced inflammation in IM\u2010HRECs induced by HG. The proposed mechanism involves targeting of the KDM3A 3\u2032 UTR by miR\u2010128\u20103p, leading to reduced KDM3A expression, while KDM3A increased NLRP3 expression by reducing H3K9me2. In conclusion, upregulation of miR\u2010128\u20103p increases the histone H3K9me2 level by inhibiting KDM3A expression, thereby reducing NLRP3 expression and suppressing DR inflammatory injury.<\/jats:p>","DOI":"10.1002\/kjm2.70120","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T04:08:33Z","timestamp":1758859713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Involvement of the\n                    <scp>miR<\/scp>\n                    \u2010128\u20103p\/\n                    <scp>KDM3A<\/scp>\n                    \/\n                    <scp>NLRP3<\/scp>\n                    Axis in High Glucose\u2010Induced Inflammatory Injury in Retinal Endothelial Cells"],"prefix":"10.1002","volume":"42","author":[{"given":"Wei\u2010Ming","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Dongguan Tungwah Hospital  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Bo","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Dongguan SongShan Lake Tungwah Hospital  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin\u2010Sheng","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Dongguan SongShan Lake Tungwah Hospital  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Dongguan SongShan Lake Tungwah Hospital  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Dongguan SongShan Lake Tungwah Hospital  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8530-1057","authenticated-orcid":false,"given":"Zhu\u2010Sheng","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Dongguan Tungwah Hospital  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061816"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2011.05.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/582060"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.564077"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-21973"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802614RRR"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12010123"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-021-00867-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2014.07.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.685466"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000001039"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11601"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/med-2021-0222"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00207454.2024.2312998"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07777"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2016.12.007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.870999"},{"issue":"3","key":"e_1_2_8_19_1","first-page":"1265","article-title":"KDM3A Inhibition Attenuates High Concentration Insulin\u2010Induced Vascular Smooth Muscle Cell Injury by Suppressing MAPK\/NF\u2010kappaB Pathways","volume":"41","author":"Zhang B. 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