{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:24Z","timestamp":1786978164628,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sepsis is typified by organ failure due to an unchecked host reaction to infection. This study aims to explore the mechanism of ubiquitin\u2010specific peptidase 7 (USP7) in sepsis with the involvement of intercellular adhesion molecule\u20101 (ICAM\u20101). A sepsis model was established using lipopolysaccharide (LPS) induction in WT and USP7\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, and various assays were conducted to evaluate survival rates, organ damage, inflammatory markers, and protein interactions. The results revealed that USP7 expression increased in LPS\u2010treated WT mice, and its interaction with ICAM\u20101 stabilized ICAM\u20101 through deubiquitination. USP7 knockout significantly elevated survival rates of septic mice. USP7 knockout reduced pulmonary inflammation, neutrophil extracellular trap (NET) formation, and myeloperoxidase and Cit\u2010H3 levels in septic mice. Moreover, USP7 knockout lowered the levels of organ injury markers (creatine kinase\u2010MB [CK\u2010MB], troponin\u2010I, and blood urea nitrogen [BUN]), liver enzymes (ALT and AST), and inflammatory markers (TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106, and IL\u20108). Co\u2010culture of bone marrow\u2010derived macrophages (BMDMs) from WT mice with ICAM\u20101+ neutrophils elevated levels of TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106. These findings suggest that USP7 plays a critical role in driving sepsis\u2010induced NET formation and inflammation by stabilizing ICAM\u20101. Targeting USP7 may represent a potential therapeutic approach to mitigate sepsis\u2010related inflammation and organ damage.\n                  <\/jats:p>","DOI":"10.1002\/kjm2.70133","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T12:14:38Z","timestamp":1761912878000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Deubiquitinase\n                    <scp>USP7<\/scp>\n                    Regulates Neutrophil Extracellular Trap Formation and Inflammation in Lipopolysaccharide\u2010Treated Mice Through\n                    <scp>ICAM<\/scp>\n                    \u20101 Expression"],"prefix":"10.1002","volume":"42","author":[{"given":"Hui","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Emergency Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Xian","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Emergency Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Fu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Emergency Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2080-7352","authenticated-orcid":false,"given":"Jiao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Emergency Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"67","article-title":"Sepsis Definition: What's New in the Treatment Guidelines","volume":"61","author":"Srzic I.","year":"2022","journal-title":"Acta Clinica Croatica"},{"issue":"7","key":"e_1_2_8_3_1","first-page":"409","article-title":"Sepsis: Diagnosis and Management","volume":"101","author":"Gauer R.","year":"2020","journal-title":"American Family 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