{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:37:44Z","timestamp":1791279464112,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the effects of puerarin and adipose\u2010derived stem cells (ADSCs), alone or combined, on p38MAPK activity, alveolar bone preservation, and inflammatory responses in a rat periodontitis (PD) model and in\u00a0vitro. ADSCs were exposed to various puerarin concentrations to assess cell proliferation, osteogenic differentiation, and p38MAPK\u2010related protein expression. Additional experiments employed anisomycin (a p38 MAPK activator) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    LPS (Pg\u2010LPS) to determine whether puerarin attenuates p38MAPK overactivation and reduces pro\u2010inflammatory cytokine production. In\u00a0vivo, ligature\u2010 and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2013induced periodontitis rats were randomized to Normal, PD, PD\u2009+\u2009ADSCs, PD\u2009+\u2009puerarin, or PD\u2009+\u2009puerarin + ADSCs groups, and alveolar bone microarchitecture (micro\u2010CT) and periodontal p38MAPK activation and osteogenic\/inflammatory proteins (Western blot, ELISA) were assessed. At 10\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u2009M, puerarin significantly increased ADSC proliferation and osteogenic differentiation, whereas anisomycin activation diminished these benefits, which were restored by co\u2010treatment with puerarin. Puerarin also reduced Pg\u2010LPS induced secretion of pro\u2010inflammatory cytokines by suppressing p38MAPK. In\u00a0vivo, the periodontitis group showed substantial alveolar bone loss and marked inflammatory cell infiltration. Both ADSCs and puerarin partially alleviated these changes but did not fully reverse them. Notably, their combination provided the greatest benefit, nearly normalizing alveolar bone parameters, strongly inhibiting p38MAPK, and further reducing inflammatory cytokine levels in periodontal tissues. Collectively, puerarin and ADSCs exert complementary anti\u2010inflammatory and pro\u2010osteogenic effects associated with attenuation of p38MAPK signaling. Co\u2010administration produced superior therapeutic outcomes, supporting this dual approach as a potential strategy for periodontitis\u2010associated bone loss.\n                  <\/jats:p>","DOI":"10.1002\/kjm2.70182","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T11:56:00Z","timestamp":1770119760000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combined Effects of Puerarin and Adipose\u2010Derived Stem Cells on Alveolar Bone Preservation and Inflammation Control in Periodontitis Through\n                    <scp>p38MAPK<\/scp>\n                    Modulation"],"prefix":"10.1002","volume":"42","author":[{"given":"Ting","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Emergency and General Dentistry Changsha Stomatological Hospital  Changsha Hunan 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