{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T14:28:31Z","timestamp":1777040911703,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ultraviolet B (UVB) radiation is a major risk factor for cataract development, but the molecular mechanisms underlying this process, particularly the involvement of regulated cell death pathways such as ferroptosis, remain unclear. Transcriptomic, proteomic, and metabolomic analyses were performed on lens tissues from UVB\u2010induced cataract rat models and controls to identify differentially expressed genes (DEGs), proteins (DEPs), and metabolites. Integrated bioinformatic analysis was then used to identify key pathways and molecules. The role of the top candidate gene, lysophosphatidylcholine acyltransferase 3 (LPCAT3), was further investigated in\u00a0vitro in rat lens epithelial cells (LECs) exposed to UVB and in\u00a0vivo using an Ad5\u2010shLPCAT3 knockdown model. Multiomics analysis identified 1787 DEGs and 355 DEPs between cataractous and normal rat lenses. KEGG enrichment highlighted pathways including complement\/coagulation cascades, cell cycle, mineral absorption, and glycerophospholipid metabolism. Metabolomic analysis revealed 2332 significantly altered metabolites enriched in sphingolipid, purine, and glycerophospholipid metabolism. Integrated analysis revealed that expression of LPCAT3, a key enzyme in glycerophospholipid metabolism, was significantly upregulated. UVB irradiation of LECs induced ferroptosis, which was characterized by decreased viability, increased ROS, MDA, 4HNE, and ACSL4 expression; decreased GPX4 expression; and upregulated LPCAT3 expression. LPCAT3 knockdown significantly protected LECs against UVB\u2010induced cell death, ROS production, lipid peroxidation, and ferroptosis marker dysregulation. Ad5\u2010shLPCAT3 injection attenuated UVB\u2010induced lens opacification, decreased ROS\/MDA levels, and reversed ferroptosis\u2010related protein changes in\u00a0vivo. This study demonstrated that LPCAT3 upregulation drives ferroptosis in LECs and that targeting LPCAT3 effectively mitigates UVB\u2010induced LEC ferroptosis and cataract formation in\u00a0vivo, highlighting its therapeutic potential.<\/jats:p>","DOI":"10.1002\/kjm2.70219","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T12:11:52Z","timestamp":1777032712000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>LPCAT3<\/scp>\n                    as a Potential Drug Target for Ultraviolet Radiation\u2013Induced Cataract: Insights From Multiomics Analysis"],"prefix":"10.1002","author":[{"given":"Fei","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Guangxi Medical University  Nanning China"},{"name":"Department of Ophthalmology Liuzhou People's Hospital Affiliated to Guangxi Medical University  Liuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Bo","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Liuzhou People's Hospital Affiliated to Guangxi Medical University  Liuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Shun","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"issue":"11","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1038\/s41433-024-02961-1","article-title":"Global Estimates on the Number of People Blind or Visually Impaired by Cataract: A Meta\u2010Analysis From 2000 to 2020","volume":"38","author":"Vision Loss Expert Group of the Global Burden of Disease Study","year":"2024","journal-title":"Eye (London, England)"},{"issue":"12","key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1080\/15384101.2023.2215084","article-title":"Ultraviolet B Radiation Induces Oxidative Stress and Apoptosis in Human Lens Epithelium Cells by Activating NF\u2010\u03baB Signaling to Down\u2010Regulate Sodium Vitamin C Transporter 2 (SVCT2) Expression","volume":"22","author":"Guo C.","year":"2023","journal-title":"Cell Cycle"},{"issue":"1","key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.2174\/0113862073365864250312043532","article-title":"Identification of the Role of Necroptosis\u2010Related Genes in the Oxidative Damage of Lens Epithelial Cells and Validation in Ultraviolet B\u2010Induced Cataract in Rats","volume":"29","author":"Liang Y.","year":"2026","journal-title":"Combinatorial Chemistry & High Throughput Screening"},{"issue":"1","key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1097\/ICU.0000000000000912","article-title":"Management of Inflammation After the Cataract Surgery","volume":"34","author":"Grzybowski A.","year":"2023","journal-title":"Current Opinion in Ophthalmology"},{"issue":"10","key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.7150\/thno.93864","article-title":"Lens Autophagy Protein ATG16L1: A Potential Target for Cataract Treatment","volume":"14","author":"Cui Y.","year":"2024","journal-title":"Theranostics"},{"issue":"7","key":"e_1_2_8_7_1","doi-asserted-by":"crossref","DOI":"10.1002\/jbio.201700377","article-title":"Ultraviolet Radiation Oxidative Stress Affects Eye Health","volume":"11","author":"Ivanov I. 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