{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:10:33Z","timestamp":1790809833073,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cerebral ischemia\u2013reperfusion injury (CI\/RI) is a major cause of secondary neuronal damage following ischemic stroke. This study investigated whether terazosin (TZ) exerts neuroprotective effects by regulating mitophagy and the reactive oxygen species (ROS)\/NOD\u2010like receptor protein 3 (NLRP3) inflammasome axis. A mouse model of CI\/RI was established and treated with TZ alone or in combination with the mitophagy inhibitor Mdivi\u20101 or the NLRP3 activator nigericin. Neurological function, infarct volume, histopathological changes in the hippocampal CA1 region, mitophagy (Mito\u2010Tracker\n                    <jats:sup>+<\/jats:sup>\n                    LC3B\n                    <jats:sup>+<\/jats:sup>\n                    ), and neuronal pyroptosis (NeuN\n                    <jats:sup>+<\/jats:sup>\n                    GSDMD\u2010N\n                    <jats:sup>+<\/jats:sup>\n                    ) were evaluated. In parallel, an oxygen\u2013glucose deprivation\/reperfusion (OGD\/R) model was established in HT\u201022 cells to assess cell viability, cytotoxicity, mitochondrial membrane potential, ROS production, inflammatory cytokine release, and expression of autophagy\u2010 and pyroptosis\u2010related proteins. The CI\/RI exhibited was characterized by worsened neurological deficits, increased infarct volume, enhanced neuronal pyroptosis, and elevated interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u201018, ROS, p62, and pyroptosis proteins, as well as prominent vacuolation and edema in the hippocampal CA1 region. These changes were accompanied by reduced mitophagy and decreased expression of LC3B II\/I and Beclin\u20101. TZ treatment markedly ameliorated these abnormalities. Mechanistic analyses showed that TZ inhibited OGD\/R\u2010induced neuronal pyroptosis by promoting mitophagy, which reduced ROS accumulation and subsequently suppressed NLRP3 inflammasome activation. Importantly, blockade of mitophagy or activation of NLRP3 weakened the protective effects of TZ. Collectively, these findings indicate that TZ mitigates CI\/RI\u2010induced neuronal injury by enhancing mitophagy and inhibiting ROS\/NLRP3\u2010dependent pyroptosis.\n                  <\/jats:p>","DOI":"10.1002\/kjm2.70242","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:16:44Z","timestamp":1781072204000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Terazosin Attenuates Neuronal Pyroptosis by Regulating the Mitochondrial\n                    <scp>ROS<\/scp>\n                    \/\n                    <scp>NLRP3<\/scp>\n                    Inflammasome Axis Through Mitophagy in Cerebral Ischemia\u2013Reperfusion Injury"],"prefix":"10.1002","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Jinan University  Guangzhou Guangdong China"},{"name":"Department of Neurology, Neuromedical Center The University of Hong Kong\u2010Shenzhen Hospital  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Yue","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shenzhen University General Hospital  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010An","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Jinan University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Sheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Jinan University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Jinan University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5747-1216","authenticated-orcid":false,"given":"An\u2010Ding","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Jinan University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"issue":"3","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2024.118982","article-title":"Drug Pairs of Huangqi and Dnggui Alleviates Pyroptosis by Promoting Autophagy Activity via AMPK\/mTOR Signaling Pathway in Middle\u2010Cerebral Artery Occlusion\/Reperfusion in Rats","volume":"337","author":"Liu L.","year":"2025","journal-title":"Journal of Ethnopharmacology"},{"issue":"12","key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"3525","DOI":"10.1007\/s11064-022-03697-8","article-title":"Cell Death Mechanisms in Cerebral Ischemia\u2010Reperfusion Injury","volume":"47","author":"Zhang Q.","year":"2022","journal-title":"Neurochemical Research"},{"issue":"3","key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1007\/s12035-017-0503-9","article-title":"Mitochondrial Quality Control and Disease: Insights Into Ischemia\u2010Reperfusion Injury","volume":"55","author":"Anzell A. 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