{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T03:44:20Z","timestamp":1786160660469,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Aspartyl\u2010tRNA synthetase 2 (DARS2), the mitochondrial enzyme responsible for aminoacylation of aspartyl\u2010tRNA, is traditionally known for its effect on protein synthesis. Recently, DARS2 is reported to be implicated in tumorigenesis. However, its specific effect on breast cancer (BC) is poorly understood. The present work employed bioinformatics tools for analyzing DARS2 expression among BC patients and its association with clinicopathological characteristics and patient prognosis. We evaluated DARS2 expression within BC cells and tissues and assessed how DARS2 knockdown or PI3K pathway activation affected cell growth, migration, invasion, apoptosis, as well as cell cycle progression. Mechanistically, we analyzed how DARS2 regulated the PI3K\/Akt\/GSK\u20103\u03b2\/\u03b2\u2010catenin pathway. Additionally, a xenograft tumor model was constructed to validate our in\u00a0vitro findings. According to our observations, DARS2 expression significantly increased in BC cells and tissues. Knockdown of DARS2 markedly suppressed cell growth, invasion, migration, and epithelial\u2010mesenchymal transition (EMT), but enhanced their apoptosis. Mechanistic studies revealed that DARS2 knockdown suppressed the PI3K\/Akt\/GSK\u20103\u03b2\/\u03b2\u2010catenin pathway, whereas PI3K pathway activation with 740Y\u2010P reversed the impacts of DARS2 knockdown. In\u00a0vivo experimental results further verified that DARS2 suppression significantly suppressed tumor growth and metastasis through downregulating the PI3K\/Akt\/GSK\u20103\u03b2\/\u03b2\u2010catenin pathway. Collectively, these results indicate that DARS2 promotes the growth, migration, invasion, and EMT of BC cells by regulating the PI3K\/Akt\/GSK\u20103\u03b2\/\u03b2\u2010catenin signaling pathway.<\/jats:p>","DOI":"10.1002\/kjm2.70267","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T03:38:38Z","timestamp":1786160318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>DARS2<\/scp>\n                    Promotes Tumorigenesis and Metastasis by Activating\n                    <scp>PI3K<\/scp>\n                    \/\n                    <scp>Akt<\/scp>\n                    \/\n                    <scp>GSK<\/scp>\n                    \u20103\u03b2\/\u03b2\u2010Catenin Signaling Pathway in Breast Cancer"],"prefix":"10.1002","author":[{"given":"Na","family":"Wang","sequence":"first","affiliation":[{"name":"School of Stomatology and Ophthalmology Xianning Medical College, Hubei University of Science and Technology  Xianning Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2833-6887","authenticated-orcid":false,"given":"Ming","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Thyroid and Breast Surgery Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, The Clinical Research Center for Acute Myocardial Infarction of Hubei Province  Xianning Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"issue":"5","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.6004\/jnccn.2024.0035","article-title":"Breast Cancer, Version 3.2024, NCCN Clinical Practice Guidelines in Oncology","volume":"22","author":"Gradishar W. 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