{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:13:54Z","timestamp":1761894834899},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T00:00:00Z","timestamp":1234137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective:<\/jats:title><jats:p>Genetic alterations, such as microsatellite instability (MSI) and loss of heterozygosity (LOH), have been detected in various inflammatory diseases, providing evidence that acquired somatic mutations might play a role in the aetiopathogenesis of chronic inflammatory conditions. The aim of this study is to assess the presence of MSI and\/or LOH in nasal cytology of patients with nasal polyps.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Prospective case\u2010controlled basic science experiment utilizing human blood and human nasal brush samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Nasal brush samples and peripheral blood from 12 patients with nasal polyps were analyzed. DNA was extracted and analyzed for MSI and LOH using the following microsatellite markers: D2S2113, D6S344, D6S1002, D11S1253, D11S480, USAT24G1, and D13S273, harboring potential susceptibility genes for nasal polyposis. Microsatellite DNA analysis was also performed in 7 control subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>MSI or LOH were revealed in 3 specimens of the nasal polyps group. Among these there were 2 cases of LOH, one for marker D11S1273 and one for D13S273, and one case of MSI in marker USAT24G1. Each one of these alterations was detected in a different patient. None of the control subjects exhibited any genetic alterations in the 7 markers tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>This is the first time that microsatellite genetic alterations are reported in nasal disease. The presence of such alterations suggests that acquired genomic somatic mutations might play a role in the pathogenesis of nasal polyps. Laryngoscope, 2009<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.20113","type":"journal-article","created":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T16:46:10Z","timestamp":1234197970000},"page":"751-756","source":"Crossref","is-referenced-by-count":3,"title":["Microsatellite DNA instability in nasal polyposis"],"prefix":"10.1002","volume":"119","author":[{"given":"Alexander D.","family":"Karatzanis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleni","family":"Tzortzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina D.","family":"Samara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eirini","family":"Neofytou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Zenk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Iro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos","family":"Siafakas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George A.","family":"Velegrakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,9]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"AndrewsAE BrysonJM Rowe\u2010JonesJM.Site of origin of nasal polyps: relevance to pathogenesis and management.2005;43:180\u2013184."},{"key":"e_1_2_6_3_2","first-page":"1","article-title":"European position paper on rhinosinusitis and nasal polyps","volume":"18","author":"Fokkens WJ","year":"2005","journal-title":"Rhinol Suppl"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01323.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e3282f363f1"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.133.11.1097"},{"key":"e_1_2_6_7_2","first-page":"702","article-title":"Co\u2010expression of the TGF\u2010beta1 and TGF\u2010beta2 isoforms in nasal polyps and in healthy mucosa","volume":"61","author":"Rostkowska\u2010Nadolska B","year":"2007","journal-title":"Postepy Hig Med Dosw (Online)"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016480701200277"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.3021"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.3037"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31811edd4f"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.01.023"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.2968"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e3180383651"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.133.7.705"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.3015"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2006.11.033"},{"key":"e_1_2_6_18_2","first-page":"125","article-title":"Nasal polyposis: is there an inheritance pattern? A single family study","volume":"46","author":"Delagranda A.","year":"2008","journal-title":"Rhinology"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(02)00052-4"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.06.00127305"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2005.05.015"},{"key":"e_1_2_6_22_2","first-page":"29","article-title":"On the essence of \u201cmeaningless\u201d simple repetitive DNA in eukaryote genomes","volume":"67","author":"Epplen C","year":"1993","journal-title":"EXS"},{"key":"e_1_2_6_23_2","first-page":"72","article-title":"Selection against frame shift mutations limits microsatellite expansion in coding DNA","volume":"10","author":"Metzgar D","year":"2000","journal-title":"Genome Res"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02905224"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.2956"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00010503"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.116.1.47"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.2214"},{"key":"e_1_2_6_29_2","first-page":"661","article-title":"Microsatellite DNA instability in nasal cytology of COPD patients","volume":"17","author":"Karatzanis AD","year":"2007","journal-title":"Oncol Rep"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1080\/000164800760370873"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(88)90030-0"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.12.1134"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.001af.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.04.052"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.09.042"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e3282fb2885"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.03.002"},{"key":"e_1_2_6_38_2","first-page":"191","article-title":"PAF in human respiratory diseases","volume":"5","author":"Furukawa M","year":"1992","journal-title":"J Lipid Mediat"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001140200"},{"key":"e_1_2_6_40_2","doi-asserted-by":"crossref","first-page":"5932","DOI":"10.3748\/wjg.v12.i37.5932","article-title":"Complementary analysis of microsatellite tumor profile and mismatch repair defects in colorectal carcinomas","volume":"12","author":"Blanes A","year":"2006","journal-title":"World J Gastroenterol"},{"key":"e_1_2_6_41_2","first-page":"221","article-title":"Loss of heterozygosity and microsatellite instability in non\u2010neoplastic mucosa from patients with chronic ulcerative colitis","volume":"2","author":"Park WS","year":"1998","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00422.2001"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01228.x"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200407000-00013"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.20113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T20:46:26Z","timestamp":1696797986000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.20113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,9]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1002\/lary.20113"],"URL":"https:\/\/doi.org\/10.1002\/lary.20113","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2,9]]}}}