{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T09:49:34Z","timestamp":1792057774993,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T00:00:00Z","timestamp":1242259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health Grant","doi-asserted-by":"crossref","award":["#R01 DC007437"],"award-info":[{"award-number":["#R01 DC007437"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010559","name":"Children's Hospital of Pittsburgh","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010559","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>To characterize the activation of cyclooxygenase (COX)\u20102\/prostaglandin (PG) E2 signaling during airway mucosal repair and its subsequent role during the wound healing process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Prospective animal study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>The subglottis was approached via cricothyroidotomy. Sham airways were closed, and wounded airways were subjected to laser injury and closed. Subglottic tissue was harvested at 12 hours, 24 hours, 48 hours, and 72 hours postinjury. Secretions were collected preoperatively and at time of sacrifice. Inflammatory gene expression was analyzed using quantitative reverse transcriptase polymerase chain reaction. Subglottic\/tracheal explants were exposed to exogenous IL\u20101\u03b2 in the presence or absence of COX inhibitors. Explant\u2010produced PGE2 levels were assayed using enzyme linked immunoassays. Human airway fibroblast migration and collagen contraction were assayed in the presence or absence of prostaglandin E2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Laser injury triggers a rapid, dose\u2010dependent increase in mucosal IL\u20101\u03b2 and COX\u20102 gene expression, with an anatomical distribution proportional to the distance from the site of injury. Gene upregulation correlates with dose\u2010dependent increases in PGE2 mucosal secretion levels. Ex vivo analysis indicates IL\u20101\u03b2 is responsible for the activation of the COX\u20102 \/ PGE2 pathway. Prostaglandin E2 differentially inhibits airway fibroblast migration and contraction in a specific, dose\u2010dependent manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>PGE2 is activated during mucosal inflammation and acts to decrease fibroplastic activity in the mucosal wound bed. During subglottic stenosis (SGS) development, the levels of PGE2 generated in response to injury may be insufficient to blunt the intrinsically fibroplastic phenotype of SGS fibroblasts, resulting in excessive scarring. Laryngoscope, 2009<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.20173","type":"journal-article","created":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T16:35:00Z","timestamp":1242318900000},"page":"1365-1373","source":"Crossref","is-referenced-by-count":14,"title":["Prostaglandin E2 is activated by airway injury and regulates fibroblast cytoskeletal dynamics"],"prefix":"10.1002","volume":"119","author":[{"given":"Vlad C.","family":"Sandulache","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tripti","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha Sheng","family":"Li\u2010Korotky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia Y.","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd 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