{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T06:10:51Z","timestamp":1785391851749,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T00:00:00Z","timestamp":1268870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Foundation for Fighting Blindness"},{"name":"Greater Baltimore Medical Center"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis.<\/jats:title><jats:p>To characterize the role of <jats:italic>Phr1<\/jats:italic>, a gene highly expressed in primary sensory neurons where it encodes an integral membrane protein with an N\u2010terminal pleckstrin homology domain and a C\u2010terminal transmembrane domain, in the olfactory system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>We studied the immunelocalization of the PHR1 protein in mouse olfactory epithelium both at steady state and during regeneration following methyl bromide (MeBr) exposure using scanning confocal microscopy. Additionally, we examined the electrophysiologic role of <jats:italic>Phr1<\/jats:italic> in olfaction and short\u2010term olfactory adaptation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>We found that PHR1 is abundantly and specifically expressed in olfactory neurons. It is widely distributed in punctate, vesiculated organelles throughout the cell bodies, axons, and glomeruli of primary olfactory neurons but is specifically excluded from the olfactory cilia. In the regenerating olfactory epithelium, PHR1 expression appears at 14 days following MeBr ablation coinciding with the onset of olfactory neuron maturity. Despite the abundant and specific expression throughout the olfactory neurons, mice lacking <jats:italic>Phr1<\/jats:italic> did not exhibit differences in the distribution of the components of olfactory signal transduction system, the rate of olfactory regeneration following MeBr exposure, olfactory function, or short\u2010term adaptation to odors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions.<\/jats:title><jats:p><jats:italic>Phr1<\/jats:italic> is widely and abundantly expressed throughout mature olfactory neurons and other primary sensory neurons, but its absence does not appear to affect olfactory morphology, regeneration, sensory function, or adaptation. The exact function of <jats:italic>Phr1<\/jats:italic> remains to be discovered. Laryngoscope, 2010<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.20779","type":"journal-article","created":{"date-parts":[[2010,3,20]],"date-time":"2010-03-20T22:33:16Z","timestamp":1269124396000},"page":"1002-1010","source":"Crossref","is-referenced-by-count":2,"title":["PHR1 is a vesicle\u2010bound protein abundantly expressed in mature olfactory neurons"],"prefix":"10.1002","volume":"120","author":[{"given":"Bruce","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunbin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Valle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,3,18]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.moo.0000193174.77321.39"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.1994.tb01821.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1418"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.18.2307"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/194589240301700311"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200402000-00018"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200208000-00019"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-6665(05)70363-7"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.50.35676"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201713200"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0320707"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.20.9137-9151.2004"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4633"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02424"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209305"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903590103"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200501120"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307882100"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606409200"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704140104"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200308260-00001"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.04.013"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1982.tb03764.x"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.10047"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00806.2002"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199901180-00003"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20779","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20779","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.20779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T08:10:17Z","timestamp":1696925417000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.20779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,18]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1002\/lary.20779"],"URL":"https:\/\/doi.org\/10.1002\/lary.20779","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,18]]}}}