{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:34:09Z","timestamp":1790051649204,"version":"4.0.1"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T00:00:00Z","timestamp":1259712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30772415"],"award-info":[{"award-number":["30772415"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30672295"],"award-info":[{"award-number":["30672295"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>To evaluate the effects of long\u2010term denervation on satellite cells (SCs) as myogenic stem cells in human posterior cricoarytenoid (PCA) muscle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Histological investigation of SCs and quantitative assessment of myoD and myogenin, which are two key myogenic regulatory factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>According to the course of denervation, denervated PCA muscles of 58 patients who suffered from traumatic unilateral vocal cord paralysis were divided into four groups: group A (6\u201312 months, 15 cases), group B (13\u201324 months, 17 cases), group C (25\u201336 months, 14 cases), and group D (more than 36 months, 12 cases). Normal PCA muscles (12 cases) were used as a control group. Immunofluorescence labeling was used to visualize the SCs. Transcription and protein expression levels of myoD and myogenin were assessed using real\u2010time quantitative polymerase chain reaction and Western blots, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>MyoD\u2010 and myogenin\u2010positive cells and embryonic myosin heavy chain\u2010positive myofibers were detected in group A and most of the samples of group B. Transcription levels of myoD and myogenin were highly upregulated in groups A and B, whereas groups C and D showed no significant difference as compared to control. Protein expression levels of myoD and myogenin peaked in group A, which was significantly different than group B. In contrast, no expression was observed in the other groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Activated SCs contribute to regenerative myogenesis in denervated laryngeal muscles through compensatory mechanisms. Laryngeal muscles exhibit persistent regenerative potential from the viewpoint of muscle SCs after less than 2 years of denervation. Laryngoscope, 2010<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.20796","type":"journal-article","created":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T20:41:25Z","timestamp":1259786485000},"page":"353-358","source":"Crossref","is-referenced-by-count":23,"title":["Functional modulation of satellite cells in long\u2010term denervated human laryngeal muscle"],"prefix":"10.1002","volume":"120","author":[{"given":"Chen","family":"Donghui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Shicai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Fei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jianjun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Gang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Hongliang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,12,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199612000-00015"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401329"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31816f693f"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-004-0429-7"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.10.007"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01353.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e318133a13c"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31803e8c8d"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00478-3"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02908"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-198209001-00001"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2007.09.012"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2002.tb00431.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0004.2000.570103.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-8-5"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00128.2003"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e318173e188"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940511400711"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199206000-00012"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1087"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1991.01870210091018"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20796","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20796","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.20796","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T04:45:52Z","timestamp":1700541952000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.20796"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,2]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1002\/lary.20796"],"URL":"https:\/\/doi.org\/10.1002\/lary.20796","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,2]]}}}