{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:55:49Z","timestamp":1791330949457,"version":"4.3.1"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,9,7]],"date-time":"2010-09-07T00:00:00Z","timestamp":1283817600000},"content-version":"vor","delay-in-days":98,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>The aim of this study was to provide a detailed cytological account on the healing tympanic membrane (TM) over 14 days and to complement existing research into TM wound healing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>The study is a prospective cohort study of 19 male Sprague\u2010Dawley (<jats:italic>Rattus norvegicus<\/jats:italic>) rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Rat TMs were perforated using a sterile needle and sacrificed at time points during the 14 days following perforation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The healing of the TM resembles cutaneous wound healing except that the TM is unique in the lack of a supportive matrix beneath the regenerating epithelia. This prevents the influx of reparative cells and nutrients and the in growth of the usual fibroblastic reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>TM wound healing contrasts with cutaneous wound healing in that keratinocytes are the first cells to close the wound and not the last. A keratin scaffold may not be important in the healing process. The malleus plays a crucial role in the healing of the TM and is the site of significant mitotic activity during the healing process. Migration across layers of the TM appears to account for the closure of the perforation. Laryngoscope, 2010<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.20998","type":"journal-article","created":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T02:57:16Z","timestamp":1275447436000},"page":"2061-2070","source":"Crossref","is-referenced-by-count":87,"title":["Histology of the healing tympanic membrane following perforation in rats"],"prefix":"10.1002","volume":"120","author":[{"given":"Peter Luke","family":"Santa Maria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon Leanne","family":"Redmond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus David","family":"Atlas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Ghassemifar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016488409122877"},{"key":"e_1_2_6_3_2","first-page":"297","article-title":"Ultrastructural and biochemical observations on the tympanic membrane","volume":"103","author":"Johnson FR","year":"1968","journal-title":"J Anat"},{"key":"e_1_2_6_4_2","first-page":"101","article-title":"Structure and function of the tympanic membrane: a review","volume":"49","author":"Lim DJ","year":"1995","journal-title":"Acta Otorhinolaryngol Belg"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016487009181875"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000276181"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000275593"},{"key":"e_1_2_6_8_2","first-page":"357","article-title":"An ultrastructural study of the skin of the tympanic membrane and external ear canal of the guinea pig","volume":"14","author":"Johnson A","year":"1985","journal-title":"J Otolaryngol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016486809126286"},{"key":"e_1_2_6_10_2","first-page":"21","article-title":"The keratinizing tympanic epithelium: an enigma","volume":"45","author":"Boedts D","year":"1991","journal-title":"Acta Otorhinolaryngol Belg"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016486909121576"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016486809126316"},{"key":"e_1_2_6_13_2","first-page":"127","article-title":"The tympanic membrane: a biochemical updating of structural components","volume":"49","author":"Broekaert D","year":"1995","journal-title":"Acta Otorhinolaryngol Belg"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348948909801002"},{"key":"e_1_2_6_15_2","first-page":"802","article-title":"Cytology of repair in experimental perforation of the tympanic membrane and its relationship to chronic perforations in man","volume":"71","author":"Taylor M","year":"1967","journal-title":"Trans Am Acad Ophthalmol Otolaryngol"},{"key":"e_1_2_6_16_2","first-page":"295","article-title":"The tympanic epithelium in normal and pathological conditions","volume":"32","author":"Boedts D","year":"1978","journal-title":"Acta Otorhinolaryngol Belg"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2273.1997.00006.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-6665(20)30467-9"},{"key":"e_1_2_6_19_2","first-page":"149","article-title":"Tympanic membrane perforations","volume":"49","author":"Boedts D","year":"1995","journal-title":"Acta Otorhinolaryngol Belg"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200305000-00004"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800530317"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100063490"},{"key":"e_1_2_6_23_2","first-page":"714","article-title":"An improved animal model for chronic perforation of the tympanic membrane [in German]","volume":"52","author":"Kaftan H","year":"2004","journal-title":"HNO"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100125411"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100084346"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199303000-00012"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199603000-00007"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016488809097011"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199603000-00020"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00179005"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016488009131734"},{"key":"e_1_2_6_32_2","first-page":"352","article-title":"Repair of the wounded guinea pig tympanic membrane: organization of filamentous actin and spatial cellular reorganization","volume":"17","author":"Weinberger JM","year":"1988","journal-title":"J Otolaryngol"},{"key":"e_1_2_6_33_2","first-page":"1302","article-title":"The healing process of tympanic membrane perforations","volume":"75","author":"Clawson JP","year":"1971","journal-title":"Trans Am Acad Ophthalmol Otolaryngol"},{"key":"e_1_2_6_34_2","first-page":"297","article-title":"Epithelial migration on the guinea\u2010pig tympanic membrane: the influence of perforation and ventilating tube insertion","volume":"8","author":"O'Donoghue GM","year":"1983","journal-title":"Clin Otolaryngol Allied Sci"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016489609137938"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016488609132838"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016488709134701"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016489009125162"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20998","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.20998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.20998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T04:23:52Z","timestamp":1697516632000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.20998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1002\/lary.20998"],"URL":"https:\/\/doi.org\/10.1002\/lary.20998","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6]]}}}