{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:27:41Z","timestamp":1781659661891,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,1,13]],"date-time":"2011-01-13T00:00:00Z","timestamp":1294876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH NIDCD","award":["R01 DC 09455"],"award-info":[{"award-number":["R01 DC 09455"]}]},{"DOI":"10.13039\/100000055","name":"NIDCD","doi-asserted-by":"crossref","award":["P30 DC005983"],"award-info":[{"award-number":["P30 DC005983"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>The inner ear is at risk for sensorineural hearing loss in both acute and chronic otitis media (OM), but the mechanisms underlying sensorineural hearing loss are unknown. Previous gene expression array studies have shown that cytokine genes might be upregulated in the cochleas of mice with acute and chronic OM. This finding implies that the inner ear could manifest a direct inflammatory response to OM that may cause sensorineural damage. Therefore, to better understand inner ear cytokine gene expression during OM, quantitative real\u2010time polymerase chain reaction and immunohistochemistry were used in mouse models to evaluate middle and inner ear inflammatory and remodeling cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Basic science experiment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>An acute OM model was created in Balb\/c mice by a transtympanic injection of <jats:italic>Streptococcus<\/jats:italic>\n<jats:italic>pneumoniae<\/jats:italic> in one ear; the other ear was used as a control. C3H\/HeJ mice were screened for unilateral chronic OM, with the noninfected ear serving as a control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Both acute and chronic OM caused both the middle ear and inner tissues in these two mouse models to overexpress numerous cytokine genes related to tissue remodeling (tumor necrosis factor\u2010\u03b1, bone morphogenetic proteins, fibroblast growth factors) and angiogenesis (vascular endothelial growth factor), as well as inflammatory cell proliferation (interleukin [IL]\u20101\u03b1,\u03b2, IL\u20102, IL\u20106). Immunohistochemistry confirmed that both the middle ear and inner ear tissues expressed these cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Cochlear tissues are capable of expressing cytokine mRNA that contributes to the inflammation and remodeling that occur in association with middle ear disease. This provides a potential molecular basis for the transient and permanent sensorineural hearing loss often reported with acute and chronic OM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.21349","type":"journal-article","created":{"date-parts":[[2011,1,13]],"date-time":"2011-01-13T19:25:20Z","timestamp":1294946720000},"page":"365-371","source":"Crossref","is-referenced-by-count":47,"title":["Altered expression of middle and inner ear cytokines in mouse otitis media"],"prefix":"10.1002","volume":"121","author":[{"given":"Carol J.","family":"MacArthur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Ann M.","family":"Pillers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqing","family":"Pang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Beth","family":"Kempton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis R.","family":"Trune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,1,13]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200009000-00034"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb51885.x"},{"key":"e_1_2_6_4_2","first-page":"161","article-title":"Role of the pro\u2010inflammatory cytokines tumor necrosis factor\u2010alpha, interleukin\u20101 beta, interleukin\u20106 and interleukin\u20108 in the pathogenesis of the otitis media with effusion","volume":"13","author":"Smirnova MG","year":"2002","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016480410022534"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348947808700602"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348948409300616"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.134.6.658"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01886-06"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000240170.48584.73"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.03.020"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2006.05.012"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000224527.41288.c4"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2008.07.029"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016480701387124"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.3342\/ceo.2008.1.3.117"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2003.10.003"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016489950180838"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-009-0165-z"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199809000-00008"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200404000-00006"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200303000-00003"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199412000-00005"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016489509139310"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1992.01880010057016"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348949210100511"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016480500452525"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.21349","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.21349","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.21349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T12:28:48Z","timestamp":1697459328000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.21349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,13]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1002\/lary.21349"],"URL":"https:\/\/doi.org\/10.1002\/lary.21349","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,13]]}}}