{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:42:03Z","timestamp":1790026923059,"version":"4.0.1"},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,2,16]],"date-time":"2011-02-16T00:00:00Z","timestamp":1297814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH Institutional Ruth L. Kirschstein-National Service Research Award","award":["5 T32 DC000028-19"],"award-info":[{"award-number":["5 T32 DC000028-19"]}]},{"name":"NIH","award":["K08 EB008122"],"award-info":[{"award-number":["K08 EB008122"]}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>By phage display, we have developed a novel peptide (NP41) that binds selectively to nerves following systemic administration. We evaluated the pattern of facial nerve labeling with fluorescently\u2010labeled NP41 (F\u2010NP41). We also tested whether F\u2010NP41 highlights facial nerves well enough to identify nerve stumps accurately several weeks after nerve transection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Forty\u2010seven wild\u2010type mice were studied prospectively. One surgeon performed the nerve transection, reanastomoses, and monitoring of functional recovery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Fluorescent labeling: F\u2010NP41 was administered intravenously (20 mice). Nerve labeling was studied with fluorescence microscopy. Transection and reanastomosis: the right facial nerve was transected (25 mice). Three weeks after transection, F\u2010NP41 was administered intravenously and fluorescence microscopy was used to identify the nerve stumps and reanastomosis in one group. Nerve identification and renastomosis was performed with white light in another group without F\u2010NP41. The control group underwent sham surgery. Time to nerve identification was recorded. Functional recovery was monitored for at least 8 weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>We found excellent labeling of intact and transected facial nerves following F\u2010NP41 administration. Several weeks following nerve transection, F\u2010NP41 provided accurate identification of the proximal and distal nerve stumps. Following reanastomosis, time to recovery and level of functional recovery was similar in the absence and presence of F\u2010NP41.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>We show improved visualization of facial nerves with a novel systemically applied fluorescently labeled probe. Use of F\u2010NP41 resulted in accurate identification of facial nerve stumps several weeks following transection. Functional recovery was similar with and without the use of F\u2010NP41. Laryngoscope, 2011<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.21411","type":"journal-article","created":{"date-parts":[[2011,2,16]],"date-time":"2011-02-16T19:59:06Z","timestamp":1297886346000},"page":"805-810","source":"Crossref","is-referenced-by-count":37,"title":["Improved facial nerve identification with novel fluorescently labeled probe"],"prefix":"10.1002","volume":"121","author":[{"given":"Amy P.","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Whitney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica L.","family":"Crisp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth","family":"Friedman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger Y.","family":"Tsien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quyen T.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,2,16]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2008.01.008"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1288\/00005537-199108000-00007","article-title":"Facial nerve trauma: optimal timing for repair","volume":"101","author":"Barr DM.","year":"1991","journal-title":"Laryngoscope"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1075832"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007010170114"},{"key":"e_1_2_7_6_2","unstructured":"WhitneyMA CrispJL NguyenLT et al.Fluorescent peptides can highlight peripheral nerves during surgery. 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