{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T16:32:24Z","timestamp":1778430744386,"version":"3.51.4"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,9,14]],"date-time":"2011-09-14T00:00:00Z","timestamp":1315958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>The aim of this study is to elucidate transcriptional changes that occur in response to tympanic membrane (TM) perforation in rats and to infer key genes and molecular events in the healing process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>A prospective cohort study of 393 male Sprague\u2010Dawley (<jats:italic>Rattus norvegicus<\/jats:italic>) rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Sprague\u2010Dawley rats were randomly allocated into either control or perforation groups spanning a 7\u2010day time period. Perforation groups consisted of 12\u2010hour, 24\u2010hour, 36\u2010hour, 2\u2010day, 3\u2010day, 4\u2010day, 5\u2010day, six\u2010day, and 7\u2010day time points. The left TMs of all perforation groups were perforated and the RNA extracted at the specified time point postperforation. Subsequent analysis was performed using Agilent's 4 \u00d7 44 k whole rat genome arrays (40 in total) to assess wound\u2010healing gene expression over a 7\u2010day time period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Over a 7\u2010day time course and at nine time points that encompassed the wounding and progression of healing, a total of 3,262 genes were differentially expressed. In this study the transcripts most upregulated occurred at 12 hours. These were Stefin A2 (344\u2010fold), Stefin 2 (143\u2010fold), and Natriuretic peptide precursor type B (222\u2010fold). Those most downregulated also occurred at 12 hours. These were alcohol dehydrogenase 7 (13.1\u2010fold) and gamma\u2010butyrobetaine hydroxylase (10.4\u2010fold). Results were validated by quantitative real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>The findings of this study provide a baseline against which to identify disease\u2010related molecular signatures, biomarkers, and to develop new treatments for TM conditions based on molecular evidence.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.22150","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T06:02:17Z","timestamp":1316066537000},"page":"2199-2213","source":"Crossref","is-referenced-by-count":23,"title":["Tympanic membrane wound healing in rats assessed by transcriptome profiling"],"prefix":"10.1002","volume":"121","author":[{"given":"Peter L.","family":"Santa Maria","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon 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