{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:24:44Z","timestamp":1773271484563,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,1,12]],"date-time":"2012-01-12T00:00:00Z","timestamp":1326326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>The aims of this study were to demonstrate the ability to enhance nerve regeneration by remote delivery of a viral vector to the crushed recurrent laryngeal nerve (RLN), to demonstrate the usefulness of a crushed RLN model to test the efficacy of viral gene therapy, and to discuss future potential applications of this approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Animal study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Adult Sprague\u2010Dawley rats were assigned to two groups. In the experimental group, an adeno\u2010associated viral (AAV) vector carrying a zinc\u2010finger transcription factor, which stimulates endogenous insulinlike growth factor I production (AAV2\u2010TO\u20106876vp16), was injected into the crushed RLN. In the control group, an AAV vector carrying the gene for green fluorescent protein was injected into the crushed RLN. Unilateral RLN paralysis was confirmed endoscopically. At 1 week, laryngeal endoscopies were repeated and recorded. Larynges were cryosectioned in 15\u2010\u03bcm sections and processed for acetylcholine histochemistry (motor endplates) followed by neurofilament immunoperoxidase (nerve fibers). Percentage nerve\u2010endplate contact (PEC) was determined and compared. Vocal fold motion was evaluated by blinded reviewers using a visual analogue scale (VAS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The difference between PEC on the crushed and uncrushed sides was statistically less in the experimental group (0.54 \u00b1 0.18 vs. 0.30 \u00b1 0.26,<jats:italic>P<\/jats:italic>= .0006). The VAS score at 1 week was significantly better in the experimental group (<jats:italic>P<\/jats:italic>= .002).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>AAV2\u2010TO\u20106876vp16 demonstrated a neurotrophic effect when injected into the crushed RLN. The RLN offers a conduit for viral gene therapy to the brainstem that could be useful for the treatment of RLN injury or bulbar motor neuron disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.22436","type":"journal-article","created":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T16:02:10Z","timestamp":1320681730000},"page":"349-355","source":"Crossref","is-referenced-by-count":14,"title":["Potential for promoting recurrent laryngeal nerve regeneration by remote delivery of viral gene therapy"],"prefix":"10.1002","volume":"122","author":[{"given":"Adam D.","family":"Rubin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman D.","family":"Hogikyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva 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