{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T17:53:49Z","timestamp":1765994029959},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>Biofilms might play a potential role in the pathogenesis and high recurrence rate of chronic rhinosinusitis with nasal polyposis (CRSwNP). Biofilm persistence has been thought to correlate with epithelial damage, subepithelial inflammatory cell infiltration, and tumor necrosis factor\u2010\u03b1 receptor (TNFR) expression in CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p>Case\u2010control experimental study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>A total of 36 patients with CRSwNP undergoing endoscopic sinus surgery were analyzed. The negative control group consisted of eight patients undergoing septoplasty for nasal obstruction without CRSwNP. The nasal polyps and inferior turbinate mucosa samples applied as negative controls were processed by hematoxylin\u2010eosin (HE) and Gram staining and TNFR\u2010I and TNFR\u2010II\u2013specific immunofluorescent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Biofilm was detected in 29 of 36 patients with CRSwNP and in none of the eight negative controls. Staining by HE showed strong correlation with the results of Gram staining protocol. In the biofilm\u2010positive cases, TNFR\u2010I and TNFR\u2010II displayed homogeneous pattern of significantly increased epithelial expression compared to the biofilm\u2010negative nasal polyps. In cases of biofilm absence, the expression pattern of TNF\u2010\u03b1 receptors was characterized by increased TNFR\u2010II\u2013specific immunoreaction. It was found that biofilm detectability corresponded to the integrity of nasal epithelium and to the dominant inflammatory cell type of the subepithelial layer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Persisting biofilms might increase the epithelial sensitivity against TNF\u2010\u03b1 that result in epithelium destruction. Coexistence of biofilms and increased TNFR expression might explain the inflammatory mucosal changes, functional disorders, and therapy resistance featuring CRSwNP. Laryngoscope,, 2012<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.23190","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T18:45:13Z","timestamp":1325875513000},"page":"504-510","source":"Crossref","is-referenced-by-count":14,"title":["Tumor necrosis factor\u2010\u03b1 receptor expression correlates with mucosal changes and biofilm presence in chronic rhinosinusitis with nasal polyposis"],"prefix":"10.1002","volume":"122","author":[{"given":"Tam\u00e1s","family":"Karosi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e9ter","family":"Csomor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istv\u00e1n","family":"Sziklai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.4065\/mcp.2010.0392"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3810\/pgm.2009.11.2081"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e3282fb2885"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3283423376"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/194589240501900506"},{"key":"e_1_2_6_7_2","first-page":"302","article-title":"Biofilm in nasal polyps","volume":"46","author":"Mladina R","year":"2008","journal-title":"Rhinology"},{"key":"e_1_2_6_8_2","first-page":"380","article-title":"Evidence of bacterial biofilms in nasal polyposis","volume":"5","author":"Zernotti ME","year":"2010","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940811701207"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31806009b0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archoto.2010.62"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-011-1623-x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.22190"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2010.24.3498"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.2500\/105065800779954329"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2009.06.090"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-9822(93)90009-D"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00237-9"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401189"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.570"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071924"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90486-2"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2540439.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2006.20.2936"},{"key":"e_1_2_6_25_2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.4193\/Rhino10.033","article-title":"Dexamethasone\u2010induced apoptosis of freshly isolated human nasal epithelial cells concomitant with abrogation of IL\u20108 production","volume":"48","author":"Bobic S","year":"2010","journal-title":"Rhinology"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/315694"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1001\/archoto.2009.219"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.03.002"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215109992519"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.23190","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.23190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T07:34:48Z","timestamp":1700552088000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.23190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,9]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/lary.23190"],"URL":"https:\/\/doi.org\/10.1002\/lary.23190","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,9]]}}}