{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T22:29:13Z","timestamp":1770071353956,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,5,8]],"date-time":"2012-05-08T00:00:00Z","timestamp":1336435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives\/Hypothesis:<\/jats:title><jats:p>Chronic rhinosinusitis (CRS) is a multifactorial disease that probably arises as a result of genetic diversity and environmental factors. <jats:italic>SPINK5<\/jats:italic> is a serine protease inhibitor, which is supposed to be an important regulator of epithelial barrier maintenance. The role of <jats:italic>SPINK5<\/jats:italic> polymorphisms and expression in CRS, especially in individuals with aspirin intolerance, is unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design:<\/jats:title><jats:p><jats:italic>SPINK5<\/jats:italic> single\u2010nucleotide polymorphisms (SNPs) and <jats:italic>SPINK5<\/jats:italic> expression levels were correlated with CRS without (CRSsNP) and with nasal polyps (CRSwNP), aspirin intolerance, asthma, and allergies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>One hundred four nasal tissue samples, 15 from patients with CRSsNP, 59 from patients with CRSwNP, and 30 from healthy controls of the inferior turbinate, were analyzed for their <jats:italic>SPINK5<\/jats:italic> status. Genotypes of four <jats:italic>SPINK5<\/jats:italic> single nucleotide polymorphism (SNPs; G1258A, G2475T, A2915G, and A1103G), as well as <jats:italic>SPINK5<\/jats:italic> mRNA expression levels, were determined by polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>No correlation between any <jats:italic>SPINK5<\/jats:italic> SNP and CRSsNP, CRSwNP, or allergies and asthma was observed. The heterozygous SNPs G1258A and A1103G were observed more frequently in aspirin\u2010intolerant patients; the homozygous (A\/A) genotype of SNP 1258 and the homozygous (G\/G) genotype SNP 1103 were less frequent. There was no correlation between the analyzed SNPs and the level of <jats:italic>SPINK5<\/jats:italic> expression. It was noted that in individuals with CRSwNP, aspirin intolerance, and allergies, <jats:italic>SPINK5<\/jats:italic> expression was lowered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>G1258A and A1103G polymorphisms are distinctive for the aspirin intolerance syndrome. Lowered <jats:italic>SPINK5<\/jats:italic> expression might be a contributing factor leading to CRS, and appears to be characteristic for patients suffering from aspirin intolerance and from allergies. Laryngoscope, 2012<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.23300","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T11:33:43Z","timestamp":1332502423000},"page":"1198-1204","source":"Crossref","is-referenced-by-count":15,"title":["Low <i>SPINK5<\/i> expression in chronic rhinosinusitis"],"prefix":"10.1002","volume":"122","author":[{"given":"Kai","family":"Fruth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyula","family":"Goebel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Koutsimpelas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Gosepath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Schmidtmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolf J.","family":"Mann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juergen","family":"Brieger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,8]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"485","article-title":"Gene expression analysis reveals novel altered genes in nasal polyps","volume":"3","author":"Babeto E","year":"2010","journal-title":"Mol Med Report"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng728"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.01860.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6363889"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.133"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0329"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.12.1114"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2008.22.3162"},{"key":"e_1_2_7_10_2","first-page":"7","article-title":"Outcome analysis of endoscopic sinus surgery for chronic sinusitis in patients having Samter's triad","volume":"29","author":"Amar YG","year":"2000","journal-title":"J Otolaryngol"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348941112000304"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000086075"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1159\/000027660"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-35002"},{"key":"e_1_2_7_15_2","first-page":"97","article-title":"EP3OS 2007: European position paper on rhinosinusitis and nasal polyps 2007. 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