{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:04:21Z","timestamp":1787065461824,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,9,10]],"date-time":"2012-09-10T00:00:00Z","timestamp":1347235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives\/Hypothesis:<\/jats:title>\n                    <jats:p>To determine if the spontaneous reinnervation that characteristically ensues after recurrent laryngeal nerve (RLN) injury could be selectively promoted and directed to certain laryngeal muscles with the use of neurotrophic factor (NF)\u2010secreting muscle stem cell (MSC) vectors while antagonistic reinnervation is inhibited with vincristine (VNC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design:<\/jats:title>\n                    <jats:p>Basic science investigation involving primary cell cultures, gene cloning\/transfer, and animal experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>MSC survival assays were used to test multiple individual NFs in vitro. Motoneuron outgrowth assays assessed the trophic effects of identified NF on cranial nerve X (CNX)\u2010derived motoneurons in vitro. Therapeutic NF was cloned into a lentiviral vector, and MSCs were tranduced to secrete NF. Sixty rats underwent left RLN transection injury, and at 3 weeks received injections of either MSCs (n = 24), MSCs secreting NF (n = 24), or saline (n = 12) into the left thyroarytenoid muscle complex; half of the animals in the MSC groups simultaneously received left posterior cricoarytenoid injections of VNC, whereas half of the animals received saline.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Ciliary neurotrophic factor (CNTF) had the greatest survival\u2010promoting effect on MSCs in culture. The addition of CNTF (50 ng\/mL) to CNX motoneuron cultures resulted in enhanced neurite outgrowth and branching. In the animal model, the injected MSCs fused with the denervated myofibers, immunohistochemistry demonstrated enhanced reinnervation based on motor endplate to nerve contact, and reverse transcriptase\u2010polymerase chain reaction confirmed stable CNTF expression at longest follow\u2010up (4 months) in the CNTF\u2010secreting MSC treated groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>MSC therapy may have a future role in selectively promoting and directing laryngeal reinnervation after RLN injury. Laryngoscope, 2012<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.23519","type":"journal-article","created":{"date-parts":[[2012,9,10]],"date-time":"2012-09-10T14:59:07Z","timestamp":1347289147000},"page":"2482-2496","source":"Crossref","is-referenced-by-count":31,"title":["Neurotrophic factor\u2010secreting autologous muscle stem cell therapy for the treatment of laryngeal denervation injury"],"prefix":"10.1002","volume":"122","author":[{"given":"Stacey L.","family":"Halum","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"McRae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadijeh","family":"Bijangi\u2010Vishehsaraei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Hiatt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,10]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1067\/mhn.2000.102574"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940211100803"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940010900405"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940711600110"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.21026"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)91632-B"},{"key":"e_1_2_7_8_2","first-page":"236","article-title":"Persistent adrenergic reinnervation of previously denervated muscle in cat","volume":"42","author":"Nomoto M","year":"1993","journal-title":"Electron Microsc"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21124"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890160035007"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348948409300414"},{"key":"e_1_2_7_12_2","unstructured":"MarieJP.Human bilateral laryngeal reinnervation: implications for transplantation. 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Paper presented at: American Laryngological Association Combined Otolaryngologic Sections Meeting (COSM) May 28\u201329 2009; Phoenix AZ."},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.125.3.274"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1998.9.14-2039"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200404000-00024"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200105000-00016"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31803e8c8d"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31816c438e"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348940911801210"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1385-299X(00)00037-4"},{"key":"e_1_2_7_21_2","unstructured":"McRaeBR AaronGP PatelRG HalumSL.Dissociated cultures and trophic factor effects on cranial nerve X motoneurons in vitro. 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