{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T14:24:10Z","timestamp":1705587850216},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,2,12]],"date-time":"2013-02-12T00:00:00Z","timestamp":1360627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>To investigate the allelic prevalence of resistance to clarithromycin in the DNA of clinical isolates of <jats:italic>Helicobacter pylori<\/jats:italic> obtained from biopsy specimens of patients with chronic tonsillitis by Scorpion real\u2010time polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Pathologic specimens of patients with chronic tonsillitis were used for rapid urease test, and blocks of paraffin\u2010embedded tonsillar tissue were used for McMullen staining, rapid urease test, and Scorpion real\u2010time PCR test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 103 biopsy samples were obtained from patients with chronic tonsillitis and examined for the presence of clarithromycin resistant <jats:italic>H. pylori<\/jats:italic>. Modified McMullen staining and rapid urease test were done on the all the samples. The DNA of specimens was extracted from the pathology blocks, and Scorpion real\u2010time PCR was performed on a final volume of 25 \u03bcL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 103 biopsy specimens, 22 samples were identified as infected by <jats:italic>H. pylori<\/jats:italic>, of which none were sensitive to clarithromycin. One had the A2143G genotype, and four had the A2142G genotype. Two had a mixed sensitive and the A2143G genotype, and five had a mixed sensitive and A2142G genotype. One strain had a mixed genotype of sensitive, A2143G, and A2142G.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The reported rate of resistance to clarithromycin is of great variation among <jats:italic>H. pylori<\/jats:italic> strains isolated from specimens in different countries. Our study showed that the most prevalent genotypes in our <jats:italic>H. pylori<\/jats:italic>\u2010positive specimens was A2142G followed by A2143G, which is different from reported results of allele\u2010specific genotyping of <jats:italic>H. pylori<\/jats:italic> strains isolated from gastric biopsy and may be a result of cross\u2010resistance to erythromycin and other macrolides.<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.23777","type":"journal-article","created":{"date-parts":[[2013,2,12]],"date-time":"2013-02-12T16:47:40Z","timestamp":1360687660000},"page":"1478-1482","source":"Crossref","is-referenced-by-count":6,"title":["Prevalence of clarithromycin\u2010resistant <i>Helicobacter pylori<\/i> in patients with chronic tonsillitis by allele\u2010specific scorpion real\u2010time polymerase chain reaction assay"],"prefix":"10.1002","volume":"123","author":[{"given":"Taghi","family":"Naserpour Farivar","sequence":"first","affiliation":[{"name":"Cell and Molecular Research Center Qazvin University of Medical Sciences Qazvin Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Najafipour","sequence":"additional","affiliation":[{"name":"Cell and Molecular Research Center Qazvin University of Medical Sciences Qazvin Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pouran","family":"Johari","sequence":"additional","affiliation":[{"name":"Cell and Molecular Research Center Qazvin University of Medical Sciences Qazvin Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,2,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i3.468"},{"key":"e_1_2_6_3_1","first-page":"33","article-title":"Helicobacter pylori in tonsillar and adenoid tissue and its possible role in oropharyngeal carcinogenesis","volume":"54","author":"Lukes P","year":"2008","journal-title":"Folia Biol (Praha)"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2008.06.018"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-9684(15)31471-1"},{"key":"e_1_2_6_6_1","first-page":"526","article-title":"Does Helicobacter pylori have a role in the development of chronic otitis media with effusion? A preliminary study","volume":"38","author":"Ozcan C","year":"2009","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.93760"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.387-389.2004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-007-0003-4"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.022111"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005457226341"},{"key":"e_1_2_6_12_1","first-page":"2550","article-title":"A systematic review of Helicobacter pylori: mechanism and stability an strains from clarithromycin\u2010treated patients","volume":"41","author":"Houben MH","year":"1999","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.12.2724"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.12.2621"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.477"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.5.1500-1504.2001"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.868-870.2005"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3765-3769.2002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2550"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02352-07"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.691-695.2001"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4512-4518.2004"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1256-1260.2005"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/11751"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2006.11.005"},{"issue":"3","key":"e_1_2_6_26_1","first-page":"S28","article-title":"Prevalence of single nucleotide mutation in clarithromycin resistant gene of Helicobacter pylori: a 32\u2010months prospective study by using hybridization real time polymerase chain reaction","volume":"95","author":"Hansomburana P","year":"2012","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.4103\/1319-3767.87181"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/187221312800166877"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-3955(03)00030-0"},{"key":"e_1_2_6_30_1","first-page":"308","article-title":"Antimicrobial resistance of Helicobacter pylori isolates from Iranian adults and children","volume":"9","author":"Siavoshi F","year":"2006","journal-title":"Arch Iranian Med"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.3161"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-007-0003-4"},{"key":"e_1_2_6_33_1","first-page":"1","article-title":"Treatment of Helicobacter pylori infection in Iran: low efficacy of recommended western regimens","volume":"7","author":"Malekzadeh R","year":"2004","journal-title":"Arch Iran Med"},{"key":"e_1_2_6_34_1","first-page":"174","article-title":"Genetic diversity and drug resistance of Helicobacter pylori strains in Isfahan, Iran","volume":"11","author":"Khashei R","year":"2008","journal-title":"Iranian J Basic Med Sci"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-2980.2011.00549.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v5.i3.263"},{"key":"e_1_2_6_37_1","first-page":"16:5746","article-title":"Antimicrobial susceptibility of Helicobacter pylori strains isolated from patients in Shiraz, Southern Iran","volume":"7","author":"Farshad S","year":"2010","journal-title":"World J Gastroenterol"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.23777","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.23777","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.23777","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.23777","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T12:03:42Z","timestamp":1692533022000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.23777"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,12]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/lary.23777"],"URL":"https:\/\/doi.org\/10.1002\/lary.23777","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,12]]}}}