{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T03:03:22Z","timestamp":1771383802026,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T00:00:00Z","timestamp":1375660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Early\u2010stage oral squamous cell carcinoma (OSCC) treatment is based on anatomic location, clinical TNM staging, and histological grade. It is a heterogeneous disease group. Classification of patients with OSCC by immunohistochemical analysis of established oncoproteins and evaluate disease course was our primary objective. Characterization of stage I OSCC patients in Southwest Finland was our secondary objective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Immunohistochemical analysis of tumor specimens and retrospective analysis of patient data of the patient treated in Turku University Hospital for T1N0M0 OSCC during the years 2000\u20102004.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paraffin\u2010embedded tumor specimens from 35 OSCC patients were collected and analyzed for HIF\u20101\u03b1, CD44, p16, Ki67, and podoplanin by immunohistochemistry and correlated with clinical findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Tumoral CD44 and HIF1\u2010\u03b1 expression levels, in combination, predicted 5\u2010year disease\u2010free survival. Reduced expression of CD44 and elevated expression of HIF1\u2010\u03b1 is associated with the lowest probability of disease\u2010free survival compared to the population as a whole (<jats:italic>P<\/jats:italic> &lt; .001 in Kaplan\u2010Meier analysis). Patients with grade I tumors demonstrated improved disease\u2010specific survival compared to those with grade II tumors (<jats:italic>P<\/jats:italic> = .027). No association was seen between p16 expression, Ki67 labeling index, or podoplanin expression and prognosis in our 35 specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HIF\u20101\u03b1 and CD44 immunohistochemical detection could potentially serve as a prognostic tool in therapy selection for early\u2010stage OSCC. <jats:italic>Laryngoscope<\/jats:italic>, 123:2435\u20132441, 2013<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.23888","type":"journal-article","created":{"date-parts":[[2013,8,8]],"date-time":"2013-08-08T17:39:32Z","timestamp":1375983572000},"page":"2435-2441","source":"Crossref","is-referenced-by-count":21,"title":["Prognostic markers in stage I oral cavity squamous cell carcinoma"],"prefix":"10.1002","volume":"123","author":[{"given":"Johannes","family":"Dunkel","sequence":"first","affiliation":[{"name":"Medicity Research Laboratory University of Turku Turku Finland"},{"name":"Turku Doctoral Programme of Biomedical Sciences University of Turku Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuli","family":"Vaittinen","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Turku Turku Finland"},{"name":"Department of Pathology Turku University Hospital Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reidar","family":"Gr\u00e9nman","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery Turku University Hospital Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilpo","family":"Kinnunen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery Turku University Hospital Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heikki","family":"Irjala","sequence":"additional","affiliation":[{"name":"Medicity Research Laboratory University of Turku Turku Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.06.002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25516"},{"issue":"9","key":"e_1_2_6_4_1","first-page":"CD006205","article-title":"Interventions for the treatment of oral and oropharyngeal cancers: surgical treatment","author":"Bessell A","year":"2011","journal-title":"Cochrane Database Syst 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