{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:55:43Z","timestamp":1791330943713,"version":"4.3.1"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T00:00:00Z","timestamp":1364342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Objectives\/Hypothesis<\/jats:title>To evaluate the efficacy of silk fibroin scaffolds (SFS<\/jats:styled-content>) and acellular collagen scaffolds (ACS<\/jats:styled-content>) for the repair of tympanic membrane (TM<\/jats:styled-content>) in a guinea pig acute perforation model.<\/jats:p><\/jats:sec>Study Design<\/jats:title>Experimental animal research.<\/jats:p><\/jats:sec>Methods<\/jats:title>Seventy\u2010two albino guinea pigs underwent perforation of the right TM and were divided into four experimental groups (n = 18). The perforations were repaired with SFS, ACS, and paper patch using onlay myringoplasty, or they were allowed to heal spontaneously (control). An additional group of 10 guinea pigs without perforation or scaffold was allocated as a normal TM<\/jats:styled-content> group. Guinea pigs in each experimental group (n = 6) were evaluated at 7, 14, and 28 days following surgery. TM<\/jats:styled-content> structural healing was evaluated by otomicroscopy and histology, and functional hearing was analyzed by auditory brainstem responses (ABR<\/jats:styled-content>). Prior to the study, mechanical properties of SFS<\/jats:styled-content> and ACS<\/jats:styled-content> were investigated.<\/jats:p><\/jats:sec>Results<\/jats:title>Tensile strength and elasticity of SFS<\/jats:styled-content> and ACS<\/jats:styled-content> were within the known range for human TM<\/jats:styled-content>. Based on otologic and histologic evaluation, TMs<\/jats:styled-content> treated with SFS<\/jats:styled-content> or ACS<\/jats:styled-content> showed complete closure of the perforation at an earlier stage, with a trilaminar structure and more uniform thickness compared to paper patch and control treated groups. ABR<\/jats:styled-content> assessment demonstrated that SFS or ACS treatment facilitated a faster restoration of hearing function compared to paper patch and control groups.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The results of this study show that SFS<\/jats:styled-content> and ACS<\/jats:styled-content> are effective graft materials and may be utilized as alternatives to current grafts for TM<\/jats:styled-content> repair.<\/jats:p><\/jats:sec>Level of Evidence<\/jats:title>N\/A. Laryngoscope, 123:1976\u20131982, 2013<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.23940","type":"journal-article","created":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T16:27:03Z","timestamp":1364401623000},"page":"1976-1982","source":"Crossref","is-referenced-by-count":49,"title":["Tympanic membrane repair using silk fibroin and acellular collagen scaffolds"],"prefix":"10.1002","volume":"123","author":[{"given":"Yi","family":"Shen","sequence":"first","affiliation":[{"name":"Ear Sciences Centre School of Surgery, The University of Western Australia Nedlands"},{"name":"Ear Science Institute Australia Subiaco Western Australia Australia"},{"name":"Department of Otolaryngology, Head & Neck Ningbo Lihuili Hospital (Ningbo Medical Centre) Ningbo Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Redmond","sequence":"additional","affiliation":[{"name":"Ear Sciences Centre School of Surgery, The University of 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