{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T21:54:36Z","timestamp":1776549276637,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T00:00:00Z","timestamp":1373241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Chronic Rhinosinusitis (<jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>) is accompanied by evidence of a vigorous adaptive immune response, and emerging studies demonstrate that some nasal polyps manifest a polyclonal autoantibody response. We previously found that antibodies against BP180, a component of the hemidesmosome complex and the dominant epitope in autoimmune bullous pemphigoid, were found at elevated levels in nasal polyp tissue. Given the critical role of hemidesmosomes in maintaining epithelial integrity, we sought to investigate the distribution of BP180 in nasal tissue and evaluate for evidence of systemic autoimmunity against this antigen in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Case\u2010control experimental study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression and distribution of BP180 in cultured nasal epithelial cells and normal nasal tissue were confirmed using real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>), Western immunoblotting, immunofluorescence and immunohistochemistry. Sera were collected from three groups: control, <jats:styled-content style=\"fixed-case\">CRSsNP<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">CRSwNP<\/jats:styled-content>. A commercially available <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was utilized to compare anti\u2010BP180 autoantibody levels in sera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>BP180 is expressed in nasal epithelium, but is not confined to the basement membrane as it is in human skin. In cultured nasal epithelial cells, confocal immunofluorescence showed a punctate distribution of BP180 along the basal surface, consistent with its distribution in epithelial keratinocytes. There are significantly higher levels of circulating nonpathologic anti\u2010BP180 autoantibodies in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> patients compared with normal controls (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>BP180 is more widely expressed in nasal epithelium versus skin, although it appears to play a similar role in the formation of hemidesmosomes along the basement membrane. Further investigations are ongoing to characterize the pathogenicity of the anti\u2010epithelial antibody response in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A. <jats:italic>Laryngoscope<\/jats:italic>, 123:2104\u20132111, 2013<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.24016","type":"journal-article","created":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T15:48:42Z","timestamp":1373298522000},"page":"2104-2111","source":"Crossref","is-referenced-by-count":37,"title":["A role for anti\u2010BP180 autoantibodies in chronic rhinosinusitis"],"prefix":"10.1002","volume":"123","author":[{"given":"Jill S.","family":"Jeffe","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudarshan","family":"Seshadri","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin J.","family":"Hamill","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia He","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roderick","family":"Carter","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Suh","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn E.","family":"Hulse","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Norton","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B.","family":"Conley","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh K.","family":"Chandra","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Kern","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery Northwestern University Feinberg School of Medicine Chicago Illinois U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan C. 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