{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T05:36:50Z","timestamp":1788068210282,"version":"build-2784847793"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T00:00:00Z","timestamp":1392854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Acquired laryngotracheal stenosis (ALTS) results from abnormal mucosal wound healing after laryngeal and\/or tracheal injury. Patients with ALTS often present late after significant reduction of the airway lumen and onset of symptoms. Motivated by the need for earlier detection of affected patients, we sought to investigate genetic markers for ALTS that would identify susceptible patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Pilot Case\u2010Control Study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seventy\u2010six patients were recruited, 40 patients with ALTS and 36 control patients with airway injury but without ALTS. DNA was isolated from whole blood and formalin\u2010fixed paraffin\u2010embedded specimens from patients. Custom primers were designed and the TaqMan assay employing allele\u2010specific polymerase chain reaction was used to interrogate single nucleotide polymorphisms (SNPs): rs2569190, rs1799750, and rs1800469 located in candidate genes CD14, matrix metalloproteinase\u20101 (MMP\u20101), and transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21), respectively. A logistic regression model was used to examine the association of candidate gene polymorphisms with the presence or absence of ALTS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 76 patients were successfully genotyped at the three loci of interest by optimizing the genotyping protocol. MMP\u20101 SNP rs1799750 was most significantly associated with development of ALTS (<jats:italic>P<\/jats:italic>\u2009=\u20090.005).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Identification of SNPs associated with development of ALTS will provide new experimental targets to study wound healing in human subjects. The association found in the current study between ALTS and SNP rs1799750 is being validated in a larger population examining an expanded set of relevant SNPs. Identifying patients with genetic susceptibility to ALTS and poor wound healing in the upper airway will be useful for management of patients after upper\u2010airway injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A. <jats:italic>Laryngoscope<\/jats:italic>, 124:E175\u2013E179, 2014<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.24382","type":"journal-article","created":{"date-parts":[[2013,8,14]],"date-time":"2013-08-14T14:20:34Z","timestamp":1376490034000},"source":"Crossref","is-referenced-by-count":17,"title":["Translational genomics of acquired laryngotracheal stenosis"],"prefix":"10.1002","volume":"124","author":[{"given":"Mursalin M.","family":"Anis","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery and the Temple University School of Pharmacy Philadelphia Pennsylvania U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Statistics, Fox School of Business Temple University Philadelphia Pennsylvania U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasvir","family":"Khurana","sequence":"additional","affiliation":[{"name":"Department of Pathology Temple University School of Medicine Philadelphia Pennsylvania U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Krynetskiy","sequence":"additional","affiliation":[{"name":"the Department of Pharmaceutical Sciences Temple University School of Pharmacy Philadelphia Pennsylvania U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M. S.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery and the Temple University School of Pharmacy Philadelphia Pennsylvania U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.22364"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1052-3359(03)00034-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940711600308"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01063.2006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archoto.2009.175"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2006.04.013"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-006-1758-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200206000-00017"},{"issue":"4","key":"e_1_2_7_10_1","first-page":"261","article-title":"Post\u2010intubation tracheal stenosis\u2013morphological, clinical investigations","volume":"54","author":"Papla B","year":"2003","journal-title":"Pol J Pathol"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2010.10.016"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-2-6"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2009.11.010"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-63"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2008.00552.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-010-1472-2"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/3.2.146"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.24382","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.24382","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/lary.24382","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.24382","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T15:11:28Z","timestamp":1692544288000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.24382"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,20]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1002\/lary.24382"],"URL":"https:\/\/doi.org\/10.1002\/lary.24382","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,20]]}}}