{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T15:58:52Z","timestamp":1760975932667},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T00:00:00Z","timestamp":1404691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Intravenous administration of mesenchymal stem cells (MSCs) has been recently shown to enhance functional recovery after stroke and spinal cord injury. The therapeutic properties of MSCs are attributed to their secretion of a variety of potent antiinflammatory and neurotrophic factors. We hypothesize that intravenous administration of MSCs after recurrent laryngeal nerve (RLN) injury in the rat may enhance functional recovery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Animal Research.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twelve 250\u2010gram Sprague\u2010Dawley rats underwent a controlled crush injury to the left RLN. After confirming postoperative vocal fold immobility, each rat was intravenously infused with either green fluorescent protein\u2010expressing MSCs or control media in a randomized and blinded fashion. Videolaryngoscopy was performed weekly. The laryngoscopy video recordings were reviewed and rated by a fellowship\u2010trained laryngologist who remained blinded to the intervention using a 0 to 3 scale.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 1 week postinjury, the MSC\u2010infused group showed a trend for higher average functional recovery scores compared to the control group (2.2 vs 1.3), but it did not reach statistical significance (<jats:italic>P<\/jats:italic> value of 0.06). By 2 weeks, however, both groups exhibited complete return of function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These pilot data indicate that with complete nerve transection by crush injury of the RLN in rat, there is complete recovery of vocal fold mobility at 2 weeks. At 1 week postinjury, animals receiving intravenous infusion of MSCs showed a trend for greater functional recovery, suggesting a potential beneficial effect of MSCs; however, this did not reach statistical significance. Therefore, no definite conclusions can be drawn from these data and further study is required.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A. <jats:italic>Laryngoscope<\/jats:italic>, 124:2555\u20132560, 2014<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.24798","type":"journal-article","created":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T07:45:18Z","timestamp":1404719118000},"page":"2555-2560","source":"Crossref","is-referenced-by-count":13,"title":["Intravenous mesenchymal stem cell therapy after recurrent laryngeal nerve injury: A preliminary study"],"prefix":"10.1002","volume":"124","author":[{"given":"Michael Z.","family":"Lerner","sequence":"first","affiliation":[{"name":"Section of Otolaryngology Yale University School of Medicine New Haven Connecticut U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Neuroscience and Regeneration Research Yale University School of Medicine New Haven Connecticut U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen L.","family":"Lankford","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Neuroscience and Regeneration Research Yale University School of Medicine New Haven Connecticut U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Radtke","sequence":"additional","affiliation":[{"name":"Department of Plastic Hand and Reconstructive Surgery Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffery D.","family":"Kocsis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Neuroscience and Regeneration Research Yale University School of Medicine New Haven Connecticut U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nwanmegha O.","family":"Young","sequence":"additional","affiliation":[{"name":"Section of Otolaryngology Yale University School of Medicine New Haven Connecticut U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016487409126379"},{"key":"e_1_2_6_3_1","first-page":"23","article-title":"Uber eine neue behandlungsmethode der rekurrenslahmung","volume":"18","author":"Bruening W","year":"1911","journal-title":"Verh Dtsch Laryg"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(77)90169-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1998.9.14-2039"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302665"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2009.111"},{"key":"e_1_2_6_8_1","first-page":"169","article-title":"Androgen treatment and 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