{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T18:46:23Z","timestamp":1782931583266,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T00:00:00Z","timestamp":1408665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Age\u2010related hearing loss has a genetic component, but there have been limited genetic studies in this field. Both N\u2010acetyltransferase 2 and apolipoprotein E genes have previously been associated. However, these studies have either used small sample sizes, examined a limited number of polymorphisms, or have produced conflicting results. Here we use a haplotype tagging approach to determine association with age\u2010related hearing loss and investigate epistasis between these two genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Candidate gene association study of a continuous phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated haplotype tagging single nucleotide polymorphisms in the N\u2010acetyltransferase 2 gene and the presence\/absence of the apolipoprotein E \u03b54 allele for association with age\u2010related hearing loss in a cohort of 265 Caucasian elderly volunteers from Greater Manchester, United Kingdom. Hearing phenotypes were generated using principal component analysis of the hearing threshold levels for the better ear (severity, slope, and concavity). Genotype data for the N\u2010acetyltransferase 2 gene was obtained from existing genome\u2010wide association study data from the Illumina 610\u2010Quadv1 chip. Apolipoprotein E genotyping was performed using Sequenom technology. Linear regression analysis was performed using Plink and Stata software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant associations (<jats:italic>P<\/jats:italic> value,\u2009&gt;\u20090.05) were observed between the N\u2010acetyltransferase 2 or apolipoprotein E gene polymorphisms and any hearing factor. No significant association was observed for epistasis analysis of apolipoprotein E \u03b54 and the N\u2010acetyltransferase 2 single nucleotide polymorphism rs1799930 (NAT2*6A).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found no evidence to support that either N\u2010acetyltransferase 2 or apolipoprotein E gene polymorphisms are associated with age\u2010related hearing loss in a cohort of 265 elderly volunteers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A. <jats:italic>Laryngoscope<\/jats:italic>, 125:E33\u2013E38, 2015<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.24898","type":"journal-article","created":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T13:39:50Z","timestamp":1408714790000},"source":"Crossref","is-referenced-by-count":13,"title":["No association between apolipoprotein <scp>E<\/scp> or <scp>N<\/scp>\u2010Acetyltransferase 2 gene polymorphisms and age\u2010related hearing loss"],"prefix":"10.1002","volume":"125","author":[{"given":"Piers","family":"Dawes","sequence":"first","affiliation":[{"name":"School of Psychological Sciences Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazel","family":"Platt","sequence":"additional","affiliation":[{"name":"Centre for Integrated Genomic Medical Research The University of Manchester, Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Horan","sequence":"additional","affiliation":[{"name":"Central Manchester University Hospitals NHS Foundation Trust Manchester Academic Health Science Centre, Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Ollier","sequence":"additional","affiliation":[{"name":"Centre for Integrated Genomic Medical Research The University of Manchester, Salford Royal NHS Hospital Manchester United Kingdom"},{"name":"Salford Research and Development Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Munro","sequence":"additional","affiliation":[{"name":"School of Psychological Sciences Salford Royal NHS Hospital Manchester United Kingdom"},{"name":"Central Manchester University Hospitals NHS Foundation Trust Manchester Academic Health Science Centre, Salford Royal NHS Hospital Manchester United Kingdom"},{"name":"Clinical Gerontology Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Pendleton","sequence":"additional","affiliation":[{"name":"Salford Research and Development Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony","family":"Payton","sequence":"additional","affiliation":[{"name":"Centre for Integrated Genomic Medical Research The University of Manchester, Salford Royal NHS Hospital Manchester United Kingdom"},{"name":"Salford Research and Development Salford Royal NHS Hospital Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a009713"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/AUD.0000000000000010"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67423-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200002010-00010"},{"key":"e_1_2_6_6_1","unstructured":"ShieldB. Evaluation of the social and economic costs of hearing impairment.2006.http:\/\/www.german.hear\u2010it.org\/multimedia\/Hear_It_Report_October_2006.pdf. 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Recommended procedure. Pure tone air and bone conduction threshold audiometry with and without masking and determination of uncomfortable loudness levels.2004.http:\/\/www.thebsa.org.uk\/docs\/RecPro\/PTA.pdf. 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