{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:09:47Z","timestamp":1773306587896,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T00:00:00Z","timestamp":1412035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Cochlear preconditioning with low doses of kanamycin or noise can reduce susceptibility to noise\u2010 and ototoxic drug\u2010induced hearing loss. The current study was undertaken to investigate whether a preconditioning regimen of low\u2010dose cisplatin would alter susceptibility to ototoxicity induced by a single large dose of cisplatin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>In vivo study using an animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010six Fischer 344\/NHsd rats were used in the study. The low\u2010dose regimen consisted of cisplatin (2 or 3 mg\/kg) given every 2 weeks by intraperitoneal injection. Control animals received injections of saline on the same schedule as the cisplatin injections. Four injections were done in total. Following the preconditioning interval, seven of the animals were sacrificed for hair cell analyses. The remaining 19 animals were exposed to 12 mg\/kg cisplatin by intraperitoneal infusion to induce cochlear injury. Auditory brainstem response (ABR) thresholds were measured 3 days after cisplatin, and the cochleae from the 19 animals were harvested and analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Statistical analyses revealed no threshold shifts, but mild outer hair cell losses, after the low\u2010dose regimen. ABR threshold shifts in the rats exposed to the 12 mg\/kg cisplatin dose were significantly higher at day 3 in the animals that underwent preconditioning with low\u2010dose cisplatin. Outer hair cell losses were also greater in the preconditioned animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Preconditioning with low\u2010dose cisplatin, using the protocol applied in the current experiment, created potentiation of cisplatin ototoxicity, rather than protection from it. There are numerous possible explanations for this effect that should be considered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 125:E78\u2013E83, 2015<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.24948","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T10:07:47Z","timestamp":1412071667000},"source":"Crossref","is-referenced-by-count":19,"title":["A low\u2010dose regimen of cisplatin before high\u2010dose cisplatin potentiates ototoxicity"],"prefix":"10.1002","volume":"125","author":[{"given":"Ryan T.","family":"Harrison","sequence":"first","affiliation":[{"name":"Department of Speech and Hearing Science The Ohio State University Columbus Ohio U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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