{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:58:09Z","timestamp":1777658289038,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T00:00:00Z","timestamp":1422921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>The direct study of human phonation is limited by the invasive and painful nature of human laryngeal neuromuscular manipulation. As a platform for the study of human phonation, indirect models have been utilized for decades such as animal, cadaveric, and computational. We sought to develop a research method allowing direct scientific control of virtually living larynges to expand our ability to understand human phonation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Canine and porcine models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nineteen canine larynges were surgically removed and reperfused with progressively adapting methodologies to create ex vivo phonation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Full neuromuscular stimulation and phonation were ultimately achieved in the ex vivo larynx. As compared with alternative perfusate solutions, heparinized whole blood was found to result in the most robust neuromuscular response. Modification of the reperfusion technique from a continuous flow to a pulsatile pump system resulted in dramatic increases in neuromuscular response and longevity of the organ. The experimental findings were repeated to demonstrate reliability of the ex vivo model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The ex vivo larynx model is demonstrated to be a repeatable platform for phonatory research. The process of development has been comprehensively described in the present report. Although the described experimental model was designed for phonatory research, this model can be readily adapted for investigations of organ transplant preservation techniques, effects of organ ischemia, and neuromuscular reinnervation capabilities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 125:1414\u20131419, 2015<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.25149","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T14:29:15Z","timestamp":1422973755000},"page":"1414-1419","source":"Crossref","is-referenced-by-count":5,"title":["Development of the ex vivo laryngeal model of phonation"],"prefix":"10.1002","volume":"125","author":[{"given":"N. Scott","family":"Howard","sequence":"first","affiliation":[{"name":"Division of Otolaryngology\u2013Head and Neck Surgery San Antonio Military Medical Center San Antonio Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abie H.","family":"Mendelsohn","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery David Geffen School of Medicine at the University of California\u2013Los Angeles Los Angeles California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald S.","family":"Berke","sequence":"additional","affiliation":[{"name":"Department of Head &amp; Neck Surgery David Geffen School of Medicine at the University of California\u2013Los Angeles Los Angeles California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-196903000-00002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-198909000-00013"},{"key":"e_1_2_7_4_1","first-page":"292","volume-title":"Vocal Fold Physiology: Biomechanics, Acoustics and Phonatory Control","author":"Scherer RC","year":"1985"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940711600210"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940411300114"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940511400607"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0892-1997(96)80039-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.427149"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348949310201006"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.2225682"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.3644913"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0892-1997(98)80034-9"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.3695403"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940711601113"},{"issue":"3","key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"C598","DOI":"10.1152\/ajpcell.1992.263.3.C598","article-title":"Regulation of oxygen consumption in fast\u2010 and slow\u2010twitch muscle","volume":"263","author":"Kushmerick MJ","year":"1992","journal-title":"Am J Physiol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2007.72"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199308000-00006"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.4776776"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.25149","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.25149","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T17:37:34Z","timestamp":1696354654000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.25149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,3]]},"references-count":18,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1002\/lary.25149"],"URL":"https:\/\/doi.org\/10.1002\/lary.25149","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,3]]}}}