{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:20:53Z","timestamp":1792624853063,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T00:00:00Z","timestamp":1427328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>To examine modulation of M\u2010cadherin, a marker for satellite cells (SCs); and MyoD, which may indicate the myogenic activity following recurrent laryngeal nerve (RLN) denervation and immediate reinnervation; and to elucidate the correlation between their modulations and establishment of neuromuscular junctions (NMJs) in the reinnervated rat thyroarytenoid (TA) muscle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Quantitative real\u2010time polymerase chain reaction qPCR and histologic assessment of the TA muscle following RLN transection and anastomosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats were divided into three groups: 1) denervation alone (DNV) (n\u2009=\u200960), 2) denervation with anastomosis (ANS) (n\u2009=\u200960), and 3) sham\u2010operated controls (n\u2009=\u200912). Animals were sacrificed at 3 days and 1, 3, and 5 weeks after treatment. TA muscles harvested from 40 animals from each DNV and ANS group; all of sham group were subjected to qPCR for assessment of the modulation of M\u2010cadherin and MyoD; and the remaining larynges of DNV and ANS group were used for histologic analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression levels of messenger RNAs (mRNAs) encoding M\u2010cadherin and MyoD in the TA muscle of the DNV group were significantly higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) than in the control throughout the study period. These mRNA levels in the ANS group were significantly higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) at\u2009\u2264\u20091 week than in the controls but fell to control levels at\u2009\u2265\u20093 weeks. In the ANS group, recovery of muscle area and NMJs structure occurred by 3 weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data suggested that NMJ formation following reinnervation might prompt recovery of M\u2010cadherin and MyoD mRNA expression to the quiescent level of SCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>N\/A. <jats:italic>Laryngoscope<\/jats:italic>, 125:E245\u2013E251, 2015<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.25248","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T20:06:08Z","timestamp":1427400368000},"source":"Crossref","is-referenced-by-count":9,"title":["Modulation of satellite cells activity and <scp>M<\/scp>yo<scp>D<\/scp> in rat thyroarytenoid muscle after reinnervation"],"prefix":"10.1002","volume":"125","author":[{"given":"Haruka","family":"Kodama","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck 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