{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:49:04Z","timestamp":1740138544422,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T00:00:00Z","timestamp":1464652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"crossref","award":["NRF-2014R1A1A2058141"],"award-info":[{"award-number":["NRF-2014R1A1A2058141"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>The development of a simple, reliable, and cost\u2010effective animal model greatly facilitates disease treatment. We aimed to establish a rapid, simple, and reproducible live zebrafish vestibular schwannoma xenograft model for antitumor drug screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We optimized each of the following conditions for tumor cell xenografts in zebrafish larvae: larval stage, incubation temperature, and injected cell number. We used NF2\u2010\/\u2010mouse Schwann (SC4) cells and generated mCherry fluorescent protein\u2010expressing cells prior to injection into zebrafish larvae. SC4 cells were counted using a fluorescence microscope, suspended in 10% fetal bovine serum, and injected into the center of the yolk sac using a microinjection system. The injected embryos were transferred to E3 medium (for zebrafish embryos), and subsequent tumor formation was observed by fluorescence microscopy over a 5\u2010day period. To validate our model, xenografted embryos were transferred into 6\u2010well plates (5 embryos per well) and treated with everolimus, a known antitumor drug.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>mCherry fluorescent protein\u2010expressing SC4 cells were successfully grafted into the yolk sacs of zebrafish embryos without any immunosuppressant treatment. At 2 days postinjection, the xenografted cells had grown into tumor masses. The optimal speed of tumor formation depended on the larval stage (30 hpf), incubation temperature (31\u00b0C), and injected cell number (200 cells). In preliminary tests, everolimus treatment yielded a &gt; 20% reduction in the number of SC4 cells in the yolk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our in vivo model has the potential to greatly facilitate vestibular schwannoma treatment because of its speed, simplicity, reproducibility, and amenability to live imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA<jats:italic>Laryngoscope<\/jats:italic>, 126:E409\u2013E415, 2016<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.26043","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T10:56:56Z","timestamp":1464692216000},"source":"Crossref","is-referenced-by-count":3,"title":["Development of a vestibular schwannoma xenograft zebrafish model for in vivo antitumor drug screening"],"prefix":"10.1002","volume":"126","author":[{"given":"Hyun\u2010Jin","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeon Ju","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sewon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology National Health Insurance Service Ilsan Hospital Goyang Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Dae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Soonchunhyang University College of Medicine Buchoen Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok\u2010Yong","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Chonnam National University Medical School Gwangju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Woon","family":"Jung","sequence":"additional","affiliation":[{"name":"New Drug Targets Lab Gwangju Institute of Science and Technology Gwangju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In Seok","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Yonsei University College of Medicine Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199811123392011"},{"key":"e_1_2_7_3_1","first-page":"927","article-title":"Vestibular schwannomas: clinical results and quality of life after microsurgery or gamma knife radiosurgery","volume":"56","author":"Myrseth E","year":"2005","journal-title":"Neurosurgery"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.21781"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/b312875j"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(98)00126-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00725.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1202"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-2-30"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1086"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2009.04.013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2mb05501e"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130330"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(94)90091-4"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2011.07.007"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3932"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780199638086.001.0001","volume-title":"Zebrafish","author":"Nusslein\u2010Volhard C","year":"2002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2014.55.1.19"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0902579"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0b013e31817f7398"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.22392"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20471"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.031401"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb700009m"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt963"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61039-9"},{"volume-title":"The Laboratory Zebrafish","year":"2012","author":"Harper C","key":"e_1_2_7_27_1"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.26043","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.26043","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.26043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T07:10:11Z","timestamp":1718608211000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.26043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,31]]},"references-count":26,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1002\/lary.26043"],"URL":"https:\/\/doi.org\/10.1002\/lary.26043","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"type":"print","value":"0023-852X"},{"type":"electronic","value":"1531-4995"}],"subject":[],"published":{"date-parts":[[2016,5,31]]}}}