{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:08:59Z","timestamp":1790204939127,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DC 000263\u201026(RAC)"],"award-info":[{"award-number":["R01 DC 000263\u201026(RAC)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 DC005209\u201015(RAC)"],"award-info":[{"award-number":["P30 DC005209\u201015(RAC)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5T32DC000022\u201028 (KWK)"],"award-info":[{"award-number":["5T32DC000022\u201028 (KWK)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Chronic, persistent infections complicate otologic procedures utilizing implantable devices such as cochlear implants or tympanostomy tubes. These infections are thought to be due to the establishment of microbial biofilms on implant surfaces. To address this issue, we hypothesized that surface charge modification may inhibit the formation of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> biofilms on implant surfaces in vitro and in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>We evaluated the effect of surface charge modification on bacterial biofilm formation by assessing the effect of the surface charge on bacterial adhesion in vitro and bacterial persistence in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To study the effect of surface charge in vitro, the surface wells in culture plates were modified using a layer\u2010by\u2010layer polyelectrolyte assembly method. Bacterial adherence was measured at 30\u2010, 60\u2010, and 120\u2010minute intervals. To study the effect of surface charge modification in vivo, the surface of titanium microscrews was similarly modified and then surgically implanted into the dorsal calvaria of adult rats and inoculated with bacteria. Two weeks after implantation and inoculation, the number of bacteria remaining in vivo was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Surface charge modification results in a significant decrease in adherence of bacteria in vitro. Surface charge modification of titanium microscrew implants also resulted in a significant decrease in <jats:italic>P. aeruginosa<\/jats:italic> recovered 2 weeks after surgical implantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Charge modification decreases the number of bacteria adherent to a surface in vitro and decreases the risk and severity of implant infection in an in vivo rat infection model. These results have promising biomedical applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, 127:1655\u20131661, 2017<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.26499","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T21:06:38Z","timestamp":1489525598000},"page":"1655-1661","source":"Crossref","is-referenced-by-count":30,"title":["Surface charge modification decreases <i>Pseudomonas aeruginosa<\/i> adherence in vitro and bacterial persistence in an in vivo implant model"],"prefix":"10.1002","volume":"127","author":[{"given":"W. Katherine","family":"Kao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Washington University in St. Louis School of Medicine St. Louis Missouri U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia M.","family":"Gagnon","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Washington University in St. Louis School of Medicine St. Louis Missouri U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph P.","family":"Vogel","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology Washington University in St. Louis School of Medicine St. Louis Missouri U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Chole","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Washington University in St. Louis School of Medicine St. Louis Missouri U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1179\/1754762811Y.0000000025"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.7874\/jao.2015.19.3.172"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cii.365"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2006.11.005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200503000-00011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-008-8335-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2014.05.025"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1017\/S019594170000480X","article-title":"Selected aspects of the socioeconomic impact of nosocomial infections: morbidity, mortality, cost, and prevention","volume":"17","author":"Jarvis WR","year":"1996","journal-title":"Infect Control Hosp Epidemiol"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2001.1052"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-89-5-764"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra035415"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/b515219b"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.155-166.2002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00428-08"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.5301\/ijao.5000151"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.revmedmi.0000259645.20603.63"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002440"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(94)90033-7"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.05008.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3629258"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.10069"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/018622-0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01338.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.1.7"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(200005)50:2<208::AID-JBM16>3.0.CO;2-D"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008989416939"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00083-8"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7765(99)00141-1"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2005.03.016"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111143098"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28881-0"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569996"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5330.1232"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/la3034534"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.131.11.983"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn102617d"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/23\/25\/255502"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.09.013"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188628"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2011.08.001"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/am507371a"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2014.8226"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3bm00197k"},{"key":"e_1_2_7_47_1","first-page":"519","article-title":"Horizontal gene transfer\u2010emerging multidrug resistance in hospital bacteria","volume":"24","author":"Dzidic S","year":"2003","journal-title":"Acta Pharmacol Sin"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.26499","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.26499","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T06:07:53Z","timestamp":1696572473000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.26499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,14]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1002\/lary.26499"],"URL":"https:\/\/doi.org\/10.1002\/lary.26499","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,14]]}}}