{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:22:39Z","timestamp":1785860559192,"version":"3.56.0"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010836","name":"Triological Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"NIDCD","doi-asserted-by":"publisher","award":["1K23DC014758"],"award-info":[{"award-number":["1K23DC014758"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"NCI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives\/Hypothesis<\/jats:title>\n                    <jats:p>Recurrent respiratory papillomatosis (RRP) is a benign disease caused by human papillomavirus (HPV) types 6 and 11. Although a prophylactic vaccine against RRP is available, a therapeutic vaccine is needed to treat those already infected. The objective of our study was to design and test a DNA vaccine targeting HPV11 proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Preclinical scientific investigation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A DNA vaccine encoding the HPV11 E6 and E7 genes linked to calreticulin (CRT) was generated. Immunologic response to the HPV11 CRT\/E6E7 vaccine was measured by vaccinating C57BL\/6 mice via electroporation and measuring CD8\u2009+\u2009T cell responses from harvested splenocytes. A tumor cell line containing HPV11\u2010E6E7 was created, and the ability of novel DNA vaccine to control tumor growth was measured in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our vaccine generated a significant and specific CD8\u2009+\u2009T\u2010cell response against the HPV11\u2010E6aa41\u201070 peptide. The CD8\u2009+\u2009T\u2010cell responses did not recognize E7 epitopes, indicating E6 immunodominance. CD8\u2009+\u2009responses were augmented in the CRT\u2010linked vaccine compared to a control non\u2010CRT vaccine. The HPV11 CRT\/E6E7 vaccine was used to treat mice inoculated with a HPV11 E6E7 expressing tumor cell line after temporary CD3 depletion to facilitate tumor growth. Vaccinated mice had a significantly lower tumor growth rate (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.029) and smaller tumor volumes compared to control mice, indicating an augmented immunologic response in vaccinated mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A DNA vaccine targeting HPV11 E6E7 generates a specific HPV11 CD\u20108\u2009+\u2009T\u2010cell response capable of reducing the growth of HPV11\u2010expressing tumors. DNA vaccines are a promising immunologic strategy for treating RRP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>\n                      NA.\n                      <jats:italic>Laryngoscope<\/jats:italic>\n                      , 127:2713\u20132720, 2017\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/lary.26737","type":"journal-article","created":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T05:04:52Z","timestamp":1500267892000},"page":"2713-2720","source":"Crossref","is-referenced-by-count":16,"title":["Immunologic responses to a novel DNA vaccine targeting human papillomavirus\u201011 E6E7"],"prefix":"10.1002","volume":"127","author":[{"given":"Julie","family":"Ahn","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Johns Hopkins School of Medicine Baltimore Maryland U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwen","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins School of Medicine Baltimore Maryland U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien\u2010Fu","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins School of Medicine Baltimore Maryland U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard B. S.","family":"Roden","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins School of Medicine Baltimore Maryland U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzyy\u2010Choou","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins School of Medicine Baltimore Maryland U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8699-033X","authenticated-orcid":false,"given":"Simon R.","family":"Best","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Johns Hopkins School of Medicine Baltimore Maryland U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2012.03.006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0598supp-525"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00239-1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20745"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.10.032"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-016-1793-x"},{"key":"e_1_2_7_8_1","first-page":"21","article-title":"Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen","volume":"56","author":"Lin KY","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c3493"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12670"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-016-0293-9"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2015.11.026"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-5-1269"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2003.01130.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005243"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004314"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4784"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02166-10"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2010.02618.x"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.26737","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.26737","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T17:31:40Z","timestamp":1695749500000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.26737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,17]]},"references-count":18,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1002\/lary.26737"],"URL":"https:\/\/doi.org\/10.1002\/lary.26737","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.727815396.793550960","asserted-by":"object"}]},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,17]]}}}