{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T01:39:35Z","timestamp":1745458775101,"version":"3.37.3"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T00:00:00Z","timestamp":1499817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"IU School of Medicine, Department of Otolaryngology-Head and Neck Surgery Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine if 2Flute (Stryker Corporation, Kalamazoo, MI) otologic burs improve the size, cellular content, and bone healing of autologous cortical bone grafts harvested during canal wall reconstruction (CWR) tympanomastoidectomy with mastoid obliteration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Institutional review board\u2010approved prospective cohort study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human autologous cortical bone chips were harvested using various burs (4 and 6\u2009mm diameter; multiflute, and 2Flute [Stryker Corporation]) from patients undergoing CWR tympanomastoidectomy for the treatment of chronic otitis media with cholesteatoma. Bone chip size, cell counts, cellular gene expression, and new bone formation were quantified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bone chips were significantly larger when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6\u2009mm diameter (113\u2009\u00b1\u200914\u2009\u03bcm<jats:sup>2<\/jats:sup> vs. 66\u2009\u00b1\u20098\u2009\u03bcm<jats:sup>2<\/jats:sup>; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and 4\u2009mm diameter (70\u2009\u00b1\u20098\u2009\u03bcm<jats:sup>2<\/jats:sup> vs. 50\u2009\u00b1\u20093\u2009\u03bcm<jats:sup>2<\/jats:sup>; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). After 2 weeks in culture, cell numbers were significantly higher when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6\u2009mm diameter (48.7\u2009\u00b1\u20093 vs. 31.8\u2009\u00b1\u20093 cells\/\u03bcg bone; P\u2009&lt;\u20090.05) and 4\u2009mm diameter (27.6\u2009\u00b1\u20091.2 vs. 8.8\u2009\u00b1\u20091.2 cells\/\u03bcg bone; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Bone\u2010derived cells express osteoblast markers (alkaline phosphatase, osteocalcin). Cultured cells are able to form new bone in culture, and bone formation is facilitated by the presence of bone chips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Use of 2Flute (Stryker Corporation) otologic burs for human autologous cortical bone harvest results in more viable bone fragments, with larger bone chips and more osteoblasts. Future studies are needed to determine if this leads to improved bone healing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, 128:E41\u2013E46, 2018<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.26779","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T06:21:03Z","timestamp":1499840463000},"source":"Crossref","is-referenced-by-count":3,"title":["Improved autologous cortical bone harvest and viability with 2<scp>F<\/scp>lute otologic burs"],"prefix":"10.1002","volume":"128","author":[{"given":"Adam A.","family":"Roth","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Indiana University School of Medicine Indianapolis Indiana U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Ciao","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Indiana University School of Medicine Indianapolis Indiana U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Medicine Indiana University School of Medicine Indianapolis Indiana U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid S.","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Department of Medicine Indiana University School of Medicine Indianapolis Indiana U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5749-6383","authenticated-orcid":false,"given":"Rick F.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Indiana University School of Medicine Indianapolis Indiana U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0b013e3182a446da"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MLG.0000187572.99335.cc"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-367-7_22"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001665-200303000-00003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.25158"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000282106.75808.af"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s40902-016-0073-1","article-title":"Cranial bone regeneration according to different particle sizes and densities of demineralized dentin matrix in the rabbit model","volume":"38","author":"Nam JW","year":"2016","journal-title":"Maxillofac Plast Reconstr Surg"},{"key":"e_1_2_6_9_1","first-page":"498","article-title":"Influence of particle size of autogenous bone grafts on the early stages of bone regeneration: a histologic and stereologic study in rabbit calvarium","volume":"17","author":"Pallesen L","year":"2002","journal-title":"Int J Oral Maxillofac Implants"},{"key":"e_1_2_6_10_1","first-page":"163","article-title":"Failure of autologous bone\u2010assisted cranioplasty following decompressive craniectomy in children and adolescents","volume":"100","author":"Grant GA","year":"2004","journal-title":"J Neurosurg"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"586","DOI":"10.3171\/2012.2.PEDS11497","article-title":"Complications of occipital screw placement for occipitocervical fusion in children","volume":"9","author":"Hwang SW","year":"2012","journal-title":"J Neurosurg Pediatr"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3171\/SPI-07\/08\/117"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.26779","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.26779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T20:02:31Z","timestamp":1696017751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.26779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,12]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1002\/lary.26779"],"URL":"https:\/\/doi.org\/10.1002\/lary.26779","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"type":"print","value":"0023-852X"},{"type":"electronic","value":"1531-4995"}],"subject":[],"published":{"date-parts":[[2017,7,12]]}}}