{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T18:53:58Z","timestamp":1773168838658,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T00:00:00Z","timestamp":1510876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/CIRG\/1458\/2016"],"award-info":[{"award-number":["NMRC\/CIRG\/1458\/2016"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Science and Technology Program key projects","doi-asserted-by":"publisher","award":["201604020187"],"award-info":[{"award-number":["201604020187"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Motile cilia impairment is a common condition in patients with chronically inflamed airways, such as is seen in nasal polyps (NPs). The mechanism underlying this pathogenic condition is complex and not fully understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated the presence and localization of dynein axonemal heavy chain 5 (DNAH5) in motile cilia using immunofluorescence staining in paraffin\u2010embedded nasal biopsies from NPs (n = 120) and inferior turbinate mucosa (n = 35) of healthy controls. We also performed single\u2010cell staining on cytospin samples (NP = 5, control = 5). Three patterns of DNAH5 localization are defined, including pattern A (presence throughout the axoneme), pattern B (undetectable in the distal part of the axoneme), and pattern C (completely missing throughout the entire axoneme). We developed a semiquantitative scoring system for which 0 = (pattern A &gt; 70%); 1 = (patterns A + B &gt; 70%); and 2 = (pattern C \u2265 30%) in each high\u2010power field (5 fields per sample).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Based on our DNAH5 scoring system, the median (1st and 3rd quartile) score was 0.3 (0.2 and 0.4) for samples from controls, and 1.1 (0.6 and 1.6) for samples from NPs in paraffin specimens (<jats:italic>P<\/jats:italic> &lt; 0.001). The DNAH5 score had a significant positive relationship with the Lund\u2010Mackay computed tomography score (<jats:italic>r<\/jats:italic> = 0.329, <jats:italic>P<\/jats:italic> = 0.005) and was higher in patients with eosinophilic NPs (<jats:italic>P<\/jats:italic> = 0.006). For cytospin samples, the mean percentage of patterns A, B, and C were 74%, 14%, and 12% in controls, and 48%, 20%, and 32% in NPs, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest that the absence or mislocalization of DNAH5 from motile cilia is a common and potentially important pathological phenomenon in chronically inflamed airway epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, 128:E97\u2013E104, 2018<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.26983","type":"journal-article","created":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T06:28:41Z","timestamp":1510900121000},"source":"Crossref","is-referenced-by-count":22,"title":["Absence or mislocalization of DNAH5 is a characteristic marker for motile ciliary abnormality in nasal polyps"],"prefix":"10.1002","volume":"128","author":[{"given":"Qianhui","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Zhujiang Hospital, Southern Medical University Guangzhou Guangdong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2113-5858","authenticated-orcid":false,"given":"Yang","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Zhujiang Hospital, Southern Medical University Guangzhou Guangdong People's Republic of China"},{"name":"Department of Otolaryngology Head and Neck Surgery, Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong People's Republic of China"},{"name":"Department of Otolaryngology National University of Singapore, National University Health System, Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenchao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6180-3956","authenticated-orcid":false,"given":"Zhuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital Zhengzhou University Zhengzhou Henan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou Guangdong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiao Hui","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore, National University Health System, Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai Sen","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore, National University Health System, Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyu","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery the 5th Affiliated Hospital of Sun Yat\u2010sen University Zhuhai Guangdong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore, National University Health System, Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology Zhujiang Hospital, Southern Medical University Guangzhou Guangdong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore, National University Health System, Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianqing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Third Affiliated Hospital of Guangzhou Medical University Guangzhou Guangdong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. N.","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. T. 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