{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:19:50Z","timestamp":1771629590492,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research","award":["20390443"],"award-info":[{"award-number":["20390443"]}]},{"name":"Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research","award":["23791911"],"award-info":[{"award-number":["23791911"]}]},{"name":"Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research","award":["25462689"],"award-info":[{"award-number":["25462689"]}]},{"name":"Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research","award":["25861572"],"award-info":[{"award-number":["25861572"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Intranasal immunization with phosphorylcholine (PC) is known to reduce immunoglobulin (Ig)E production. However, its effects on the occurrence of allergic rhinitis (AR) are unknown. This study was performed to evaluate the effects of PC\u2010keyhole limpet hemocyanin (PC\u2010KLH) and to examine the effects on the occurrence of AR in a murine model of AR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>In vivo study using an animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010five female BALB\/c mice were divided into three groups; those pretreated with intranasal administration of PC\u2010KLH followed by intraperitoneal sensitization and nasal challenge with ovalbumin (OVA) (group A), those untreated with PC\u2010KLH followed by sensitization and nasal challenge with OVA (group B), and those untreated with PC\u2010KLH or OVA as controls (group C). Nasal symptoms, allergic inflammation in the nasal mucosa, OVA specific IgE production, and cytokine profile were compared among those three groups. Dendritic cells (DCs) were isolated from splenic cells and PC\u2010KLH\u2013stimulated interleukin (IL)\u201012p40 production was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mice pretreated with PC\u2010KLH showed lower allergic nasal symptoms and inflammation compared to untreated mice. The levels of total IgE and OVA\u2010specific IgE in serum, and IL\u20104 production by nasal and splenic CD4<jats:sup>+<\/jats:sup> T cells were significantly reduced by PC\u2010KLH pretreatment. Furthermore, IL\u201012p40 production by DCs was induced by PC\u2010KLH in a dose\u2010dependent manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Intranasal administration of PC\u2010KLH suppressed allergic inflammation in nasal mucosa and antigen\u2010specific IgE production by downregulating Th2\u2010type immune response. Intranasal immunization with PC might be useful to prevent AR and upper airway bacterial infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, 128:E234\u2013E240, 2018<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27030","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T17:38:52Z","timestamp":1511977132000},"source":"Crossref","is-referenced-by-count":4,"title":["Intranasal immunization with phosphorylcholine suppresses allergic rhinitis in mice"],"prefix":"10.1002","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2903-5699","authenticated-orcid":false,"given":"Keiichi","family":"Miyashita","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Kagoshima University Graduate School of Medical and Dental Sciences Kagoshima 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