{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:24:04Z","timestamp":1760217844827,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T00:00:00Z","timestamp":1514332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science Fund for Youths in Heilongjiang Province","award":["QC2012C040"],"award-info":[{"award-number":["QC2012C040"]}]},{"name":"Haiyan Scientific Research Fund in the Affiliated Tumor Hospital of Harbin Medical University","award":["JJMS2014-02"],"award-info":[{"award-number":["JJMS2014-02"]}]},{"name":"Post-doctoral start fund in Heilongjiang Province","award":["LBH-Z17118"],"award-info":[{"award-number":["LBH-Z17118"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>Human laryngeal squamous cell carcinoma (LSCC) is a malignancy that was discovered originally in the epithelial tissue of laryngeal mucosa. However, the underlying molecular mechanism is still not clear. In this study, we aimed to investigate the potential molecular mechanisms of TSR2 in the LSCC cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>The expression of TSR2 was first analyzed in LSCC tissues. Then functional effects of TSR2 on Hep\u20102 and AMC\u2010HN\u20108 cell lines were performed by overexpression pcDNA3.1\u2010TSR2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated the expression level of TSR2 in LSCC tissues and cells by performing quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR). The pcDNA3.1\u2010TSR2 was constructed to explore the effect of overexpressing TSR2 in Hep\u20102 cells and AMC\u2010HN\u20108 cells. We further investigated the effect of overexpressing TSR2 on cell apoptosis\u2010related protein and nuclear factor kappa\u2010light\u2010chain\u2010enhancer of activated B cells (NF\u2010\u03baB) p65 nuclear translocation through Western blot and terminal dUTP nick end\u2010labeling assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that TSR2 was downregulated in LSSC tissues and cells compared with the controls, and the overexpression of TSR2 in Hep\u20102 and AMC\u2010HN\u20108 cells could promote cell apoptosis and related apoptosis proteins. The Western blot\/qRT\u2010PCR data further indicated that overexpression of TSR2 in Hep\u20102 and AMC\u2010HN\u20108 cells could lead to a block of NF\u2010\u03baB signaling pathway via decreasing nuclear NF\u2010\u03baB p65 and increasing cytoplasm NF\u2010\u03baB p65. Moreover, overexpression of TSR2 significantly inhibited the phosphorylation of I\u03baB\u03b1 and IKK\u03b1\/\u03b2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results indicated that TSR2\u2010induced apoptosis was mediated by inhibiting the NF\u2010\u03baB signaling pathway, which may provide an effective target in gene therapy for LSCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, 128:E130\u2013E134, 2018<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27035","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T10:22:58Z","timestamp":1514370178000},"source":"Crossref","is-referenced-by-count":11,"title":["TSR2 Induces laryngeal cancer cell apoptosis through inhibiting NF\u2010\u03baB signaling pathway"],"prefix":"10.1002","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8354-0365","authenticated-orcid":false,"given":"Hong\u2010Jiang","family":"He","sequence":"first","affiliation":[{"name":"Department of Head and Neck Surgery Affiliated Tumor Hospital of Harbin Medical University Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Bing","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Hospital of Heilongjiang Province Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guijun","family":"Liu","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine Heilongjiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,27]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_6_2_1","DOI":"10.3322\/canjclin.56.2.106"},{"doi-asserted-by":"publisher","key":"e_1_2_6_3_1","DOI":"10.1016\/S0030-6665(20)30262-0"},{"doi-asserted-by":"publisher","key":"e_1_2_6_4_1","DOI":"10.1134\/S0026893311020099"},{"doi-asserted-by":"publisher","key":"e_1_2_6_5_1","DOI":"10.1111\/j.0105-2896.2006.00375.x"},{"doi-asserted-by":"publisher","key":"e_1_2_6_6_1","DOI":"10.1007\/s11010-009-0267-2"},{"doi-asserted-by":"publisher","key":"e_1_2_6_7_1","DOI":"10.1038\/onc.2010.566"},{"doi-asserted-by":"publisher","key":"e_1_2_6_8_1","DOI":"10.1126\/science.1099962"},{"doi-asserted-by":"publisher","key":"e_1_2_6_9_1","DOI":"10.1016\/j.jprot.2012.09.034"},{"doi-asserted-by":"publisher","key":"e_1_2_6_10_1","DOI":"10.1038\/sj.thj.6200252"},{"doi-asserted-by":"publisher","key":"e_1_2_6_11_1","DOI":"10.1080\/01926230701320337"},{"doi-asserted-by":"publisher","key":"e_1_2_6_12_1","DOI":"10.1093\/emboj\/17.14.3878"},{"doi-asserted-by":"publisher","key":"e_1_2_6_13_1","DOI":"10.1007\/978-1-59745-504-6_9"},{"doi-asserted-by":"publisher","key":"e_1_2_6_14_1","DOI":"10.4161\/cbt.5.7.3228"},{"doi-asserted-by":"publisher","key":"e_1_2_6_15_1","DOI":"10.1051\/jbio:2005017"},{"doi-asserted-by":"publisher","key":"e_1_2_6_16_1","DOI":"10.1016\/j.mcn.2004.10.006"},{"doi-asserted-by":"publisher","key":"e_1_2_6_17_1","DOI":"10.1038\/nature04870"},{"doi-asserted-by":"publisher","key":"e_1_2_6_18_1","DOI":"10.1126\/science.1107904"},{"doi-asserted-by":"publisher","key":"e_1_2_6_19_1","DOI":"10.1007\/s12094-008-0171-3"},{"doi-asserted-by":"publisher","key":"e_1_2_6_20_1","DOI":"10.1371\/journal.pone.0050160"},{"doi-asserted-by":"publisher","key":"e_1_2_6_21_1","DOI":"10.1126\/science.274.5291.1383"},{"doi-asserted-by":"publisher","key":"e_1_2_6_22_1","DOI":"10.1038\/sj.onc.1208842"},{"doi-asserted-by":"publisher","key":"e_1_2_6_23_1","DOI":"10.1007\/s11010-007-9440-7"},{"doi-asserted-by":"publisher","key":"e_1_2_6_24_1","DOI":"10.1126\/science.274.5288.787"},{"doi-asserted-by":"publisher","key":"e_1_2_6_25_1","DOI":"10.4049\/jimmunol.166.8.4949"},{"doi-asserted-by":"publisher","key":"e_1_2_6_26_1","DOI":"10.1182\/blood-2004-04-1463"},{"doi-asserted-by":"publisher","key":"e_1_2_6_27_1","DOI":"10.1007\/s11033-014-3634-3"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Flary.27035","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/lary.27035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T09:18:57Z","timestamp":1695547137000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/lary.27035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,27]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1002\/lary.27035"],"URL":"https:\/\/doi.org\/10.1002\/lary.27035","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"type":"print","value":"0023-852X"},{"type":"electronic","value":"1531-4995"}],"subject":[],"published":{"date-parts":[[2017,12,27]]}}}