{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:06:04Z","timestamp":1773795964820,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T00:00:00Z","timestamp":1533859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Use of cell culture and conventional in vivo mammalian models to assess nerve regeneration across guidance conduits is resource\u2010intensive. Herein we describe a high\u2010throughput platform utilizing transgenic mice for stain\u2010free axon visualization paired with rapid cryosection techniques for low\u2010cost screening of novel bioengineered nerve guidance conduit performance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Interposition repair of sciatic nerve transection in mice expressing yellow fluorescent protein in peripheral neurons (Thy1.2 YFP\u201016) was performed with various bioengineered neural conduit compositions using a rapid sutureless entubulation technique under isoflurane anesthesia. Axonal ingrowth was assessed at 3 and 6 weeks using epifluorescent microscopy following cryosectioning.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean procedure time (incision\u2010to\u2010closure) was less than 2\u00bd minutes. Direct operational costs of a 3\u2010week experiment was calculated at $21.47 per animal. Tissue processing steps were minimized to aldehyde fixation, cryoprotection and sectioning, and rapid fluorescent dye staining for conduit visualization. Fluorescent microscopy readily resolved robust axonal sprouting at 3 weeks, with clear elucidation of ingrowth\u2010permissive, semipermissive, or restrictive nerve guidance conduit environments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A rapid and cost\u2010efficient in vivo platform for screening of nerve guidance conduit performance has been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA. <jats:italic>Laryngoscope<\/jats:italic>, E392\u2013E392, 2018<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27439","type":"journal-article","created":{"date-parts":[[2018,8,11]],"date-time":"2018-08-11T05:33:03Z","timestamp":1533965583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Fluorescent Reporter Mice for Nerve Guidance Conduit Assessment: A High\u2010Throughput in vivo Model"],"prefix":"10.1002","volume":"128","author":[{"given":"Suresh","family":"Mohan","sequence":"first","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Department of Otolaryngology, Massachusetts Eye and Ear Infirmary  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iv\u00e1n Coto","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Department of Otolaryngology, Massachusetts Eye and Ear Infirmary  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjin","family":"Wang","sequence":"additional","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Department of Otolaryngology, Massachusetts Eye and Ear Infirmary  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiyang","family":"Yin","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering, Dartmouth College  Hanover New Hampshire U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathryn A.","family":"Sundback","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Department of Surgery, Massachusetts General Hospital Harvard Medical School  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike G.K.","family":"Wegst","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering, Dartmouth College  Hanover New Hampshire U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nate","family":"Jowett","sequence":"additional","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Department of Otolaryngology, Massachusetts Eye and Ear Infirmary  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2010.12.030"},{"key":"e_1_2_7_3_1","unstructured":"WegstUGK WheatleyM RiblettBW FrancisN DoniusAE inventors.Porous polymer scaffolds for neural tissue engineering and methods of producing the same. 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