{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T01:56:05Z","timestamp":1771466165909,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["#25462680"],"award-info":[{"award-number":["#25462680"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Objectives\/Hypothesis<\/jats:title><jats:p>To elucidate the aging physiology of the vocal folds, we examined the characters of aged vocal fold fibroblasts (VFFs) in various conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>In vitro study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>VFFs from young (12\u2010week\u2010old) and aged (19\u2010month\u2010old) Sprague\u2010Dawley rats were compared. Proliferative capacity, ratio of myofibroblast to fibroblast, myofibroblast function, and extracellular matrix production were examined in the following conditions: na\u00efve, basic fibroblast growth factor (bFGF) supplemented, and hepatocyte growth factor (HGF) supplemented.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Aged VFFs demonstrated reduced proliferation by cell counting, though the ratio of Ki\u201067\u2013positive cells showed no difference. Aged VFFs exhibited an increased expression of \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA); however, they demonstrated no enhanced contractile ability in a gel contraction assay. Type I collagen protein was increased age dependently, accompanied with decreased <jats:italic>Mmp1<\/jats:italic> and unchanged <jats:italic>Col1a1<\/jats:italic> transcription. Type I collagen protein and \u03b1\u2010SMA represented quite similar reduction patterns to bFGF or HGF administration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The following possible characteristics of aged VFFs were implied: long duration of mitosis, increased myofibroblast population size with certain dysfunctions, reduced type I collagen turnover, and correlation between \u03b1\u2010SMA expression and type I collagen metabolism. Further investigations of these features will help to clarify presbyphonia's pathology and establish treatment strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Level of Evidence<\/jats:title><jats:p>NA <jats:italic>Laryngoscope<\/jats:italic>, 129:E94\u2013E101, 2019<\/jats:p><\/jats:sec>","DOI":"10.1002\/lary.27464","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T10:23:20Z","timestamp":1542623000000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of aged rat vocal fold fibroblasts"],"prefix":"10.1002","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5263-2702","authenticated-orcid":false,"given":"Yoshitaka","family":"Kawai","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Graduate School of Medicine, Kyoto University  Kyoto 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